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与红树植物木榄(Bruguiera sexangula (Lour.) Poir.)相关的可培养内生真菌的系统发育多样性、抗菌和抗氧化潜力及生物活性化合物鉴定

Phylogenetic Diversity, Antimicrobial and Antioxidant Potential and Identification of Bioactive Compounds from Culturable Endophytic Fungi Associated with Mangrove Bruguiera sexangula (Lour.) Poir.

作者信息

Zhang Xu, Xu Zhiyong, Ma Jiankun, Zhou Dongdong, Xu Jing

机构信息

Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Chemical Engineering and Technology, Hainan University, Haikou, 570228, People's Republic of China.

School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, People's Republic of China.

出版信息

Curr Microbiol. 2021 Feb;78(2):479-489. doi: 10.1007/s00284-020-02314-7. Epub 2021 Jan 2.

Abstract

A total of 96 isolates were obtained from 375 segments, isolated from the healthy roots, stems, leaves, hypocotyls and flowers of Bruguiera sexangula (Lour.) Poir. collected at the Dong Zhai Gang Mangrove Garden on Hainan Island, and 20 independent representative isolates were identified using a combination of morphological and molecular approaches. The most frequent endophytic fungal species isolated were Diaporthe phaseolorum (relative frequency = 31.2%). The Shannon-Wiener diversity and Simpson's diversity index both showed that stems possessed the highest diversity compared to the other tissues estimated. Ethyl acetate extracts and the isolated metabolites were tested for antimicrobial activity using the serial dilution technique and for antioxidant activity using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radical-scavenging capacity assays, respectively. The fungal isolate HL18 (Gelasinospora endodonta) cultured on Czapek's agar (CA) displayed a broad spectrum of antimicrobial activities and was significantly active against Escherichia coli (MIC = 0.0625 mg ml). Antioxidant assays showed that most of the fungal isolates (60.0%) exhibited some degree of antioxidant capacity (%RSA > 50%). The stain HL14 (Pestalotiopsis mangiferae) grown on potato dextrose agar (PDA) exhibited the highest DPPH and ABTS radical-scavenging capability with IC values of 0.717 ± 0.012 mg ml and 0.787 ± 0.027 mg ml, respectively. Furthermore, five known secondary metabolites 1-5 were isolated and identified from HL-14. Compounds 1 and 5 exhibited weak antioxidant activity.

摘要

从海南岛东寨港红树林采集的海莲(Bruguiera sexangula (Lour.) Poir.)健康的根、茎、叶、下胚轴和花的375个组织片段中,共获得96株分离物,并采用形态学和分子学方法相结合的方式鉴定出20株独立的代表性分离物。分离出的最常见内生真菌物种是菜豆间座壳(Diaporthe phaseolorum)(相对频率 = 31.2%)。香农 - 维纳多样性指数和辛普森多样性指数均表明,与其他估计组织相比,茎的多样性最高。分别采用系列稀释技术测试乙酸乙酯提取物和分离出的代谢产物的抗菌活性,采用2,2'-二苯基-1-苦基肼(DPPH)和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除能力测定法测试其抗氧化活性。在查氏琼脂(CA)上培养的真菌分离物HL18(内生根孢菌(Gelasinospora endodonta))表现出广谱抗菌活性,对大肠杆菌具有显著活性(最低抑菌浓度(MIC)= 0.0625 mg/ml)。抗氧化测定表明,大多数真菌分离物(60.0%)表现出一定程度的抗氧化能力(%RSA > 50%)。在马铃薯葡萄糖琼脂(PDA)上生长的菌株HL14(芒果拟盘多毛孢(Pestalotiopsis mangiferae))表现出最高的DPPH和ABTS自由基清除能力,IC值分别为0.717±0.012 mg/ml和0.787±0.027 mg/ml。此外,从HL - 14中分离并鉴定出5种已知的次生代谢产物1 - 5。化合物1和5表现出较弱的抗氧化活性。

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