Liu Sisi, Hou Yage, Zheng Kaixuan, Ma Qian, Wen Meng, Shao Shicheng, Wu Shaohua
Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, China.
Department of Gardening and Horticulture, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla County, Yunnan, China.
Front Microbiol. 2024 Feb 27;15:1258208. doi: 10.3389/fmicb.2024.1258208. eCollection 2024.
exhibits high edible and medicinal value; however, there have been no reports on the exploration of its endophyte resources. Here, we conducted analyses encompassing plant metabolomics, microbial diversity, and the biological activities of endophytic metabolites in . . High-throughput sequencing identified 4,913 endophytic fungal amplicon sequence variants (ASVs) and 1,703 endophytic bacterial ASVs from the roots, stems, leaves, flowers, and fruits of . Fungi were classified into 5 phyla, 24 classes, 75 orders, 170 families, and 313 genera, while bacteria belonged to 21 phyla, 47 classes, 93 orders, 145 families, and 232 genera. Furthermore, there were significant differences in the composition and content of metabolites in different tissues of . . Spearman's correlation analysis of the differential metabolites and endophytes revealed that the community composition of the endophytes correlated with plant-rich metabolites. The internal transcribed spacer sequences of 105 isolates were determined, and phylogenetic analyses revealed that these fungi were distributed into three phyla (Ascomycota, Basidiomycota, and Mucoromycota) and 20 genera. Moreover, 16S rDNA sequencing of 46 bacteria revealed they were distributed in 16 genera in three phyla: . The antimicrobial activities (filter paper method) and antioxidant activity (DPPH and ABTS assays) of crude extracts obtained from 68 fungal and 20 bacterial strains cultured in different media were evaluated. Additionally, the α-glucosidase inhibitory activity of the fungal extracts was examined. The results showed that 88.6% of the strains exhibited antimicrobial activity, 55.7% exhibited antioxidant activity, and 85% of the fungi exhibited α-glucosidase inhibitory activity. The research suggested that the endophytes of . are highly diverse and have the potential to produce bioactive metabolites, providing abundant species resources for developing antibiotics, antioxidants and hypoglycemic drugs.
具有较高的食用和药用价值；然而，关于其内生菌资源的探索尚无报道。在此，我们进行了包括植物代谢组学、微生物多样性以及内生代谢产物生物活性等方面的分析。高通量测序从……的根、茎、叶、花和果实中鉴定出4913个内生真菌扩增子序列变体（ASV）和1703个内生细菌ASV。真菌被分为5个门、24个纲、75个目、170个科和313个属，而细菌属于21个门、47个纲、93个目、145个科和232个属。此外，……不同组织中代谢产物的组成和含量存在显著差异。对差异代谢产物和内生菌进行斯皮尔曼相关性分析表明，内生菌的群落组成与植物丰富的代谢产物相关。测定了105株分离株的内部转录间隔区序列，系统发育分析表明这些真菌分布在三个门（子囊菌门、担子菌门和毛霉门）和20个属中。此外，对46株细菌的16S rDNA测序表明它们分布在三个门的16个属中：……评估了从在不同培养基中培养的68株真菌和20株细菌菌株获得的粗提物的抗菌活性（滤纸片法）和抗氧化活性（DPPH和ABTS测定法）。此外，还检测了真菌提取物的α - 葡萄糖苷酶抑制活性。结果表明，88.6%的菌株具有抗菌活性，55.7%具有抗氧化活性，85%的真菌具有α - 葡萄糖苷酶抑制活性。该研究表明……的内生菌具有高度多样性，有产生生物活性代谢产物的潜力，为开发抗生素、抗氧化剂和降血糖药物提供了丰富的物种资源。
