State Key Laboratory of Solidification Processing, Shaanxi Engineering Laboratory for Graphene New Carbon Materials & Application, School of Material Science and Engineering, Northwestern Polytechnical University, Xian, 710072, Shaanxi, People's Republic of China.
NCP-NPU Joint International Research Center on Advanced Nanomaterials and Defects Engineering School of Material Science and Engineering, Northwestern Polytechnical University, Xian, 710072, Shaanxi, People's Republic of China.
Biotechnol Lett. 2021 Apr;43(4):781-790. doi: 10.1007/s10529-020-03071-0. Epub 2021 Jan 2.
In the present work, we propose the synthesis of novel nicotinamide subsituted phthlocyanine photosensitizer (PS) and characterized by FTIR, UV-visible, H-NMR and MALDI Toff spectroscopy. Nicotinamide plays a vital rule in the central nervous system and its potential as a therapeutic for neurodegenerative disease. Nicotinamide substituted PS (3) efficiently produced ROS via type-1 process as measured by DCF assay. We observed that our PS after red light illumination (22 J/cm) killed gram positive S. aureus upto 3 log reduction. Furher the addition of Potassium Iodide (100 mM) significantly potentiated PS at lower concentrations and enhanced the bacterial killing upto 6 log reduction against the S. aureus. We further found that the synergistic effect of PS and KI also eradicated the gram negative E. coli strain at lower concentraion of PS and found to killed E. coli upto 5 log reduction under the red light illumination at 22 J/cm of light dose. The conjugation of such biologically important form of vitamin B with PS would be a great addition and could pav the way for the novel photodynamic agent in the treatement of cancer and infectious diseases. A new symmetrical Nicotinamide tetrasubstituted zinc phthalocyanine (3) was synthesized. Upon addition of potassium Iodide with PS, the PS exhibited significant photodynamic activity with 5-6 logs reduction in bacterial load was achieved.
在本工作中，我们提出了一种新型烟酰胺取代的酞菁光敏剂（PS）的合成方法，并通过傅里叶变换红外光谱（FTIR）、紫外-可见光谱（UV-Vis）、氢核磁共振谱（H-NMR）和基质辅助激光解吸电离飞行时间质谱（MALDI Toff 光谱）进行了表征。烟酰胺在中枢神经系统中起着至关重要的作用，它有作为神经退行性疾病治疗药物的潜力。烟酰胺取代的 PS（3）通过 DCF 测定法测量，可有效地通过 1 型过程产生 ROS。我们观察到，我们的 PS 在红光照射（22 J/cm）后，能够将革兰氏阳性菌金黄色葡萄球菌的数量减少 3 个对数级。此外，加入碘化钾（100 mM）可在较低浓度下显著增强 PS 的作用，并将金黄色葡萄球菌的杀菌能力提高至 6 个对数级。我们进一步发现，PS 和 KI 的协同作用还可以在较低浓度的 PS 下消灭革兰氏阴性大肠杆菌菌株，并在 22 J/cm 的光剂量下的红光照射下，将大肠杆菌的数量减少 5 个对数级。将这种具有生物重要性的维生素 B 形式与 PS 结合，将是一个很好的补充，并为癌症和传染病的新型光动力治疗剂铺平道路。合成了一种新的对称烟酰胺四取代锌酞菁（3）。在 PS 中加入碘化钾后，PS 表现出显著的光动力活性，细菌负荷减少了 5-6 个对数级。
