Department of Dermatology, West China Hospital, Sichuan University, No.37 Guo Xue Alley, Chengdu, 610041, China.
Laboratory of Dermatology, Clinical Institute of Inflammation and Immunology, Frontiers Science Center for Disease-related Molecular Network West China Hospital, Sichuan University, Chengdu, 610041, China.
BMC Microbiol. 2024 Jul 5;24(1):246. doi: 10.1186/s12866-024-03401-6.
Previous studies have shown that antimicrobial photodynamic inactivation (aPDI) can be strongly potentiated by the addition of the non-toxic inorganic salt, potassium iodide (KI). This approach was shown to apply to many different photosensitizers, including the xanthene dye Rose Bengal (RB) excited by green light (540 nm). Rose Bengal diacetate (RBDA) is a lipophilic RB derivative that is easily taken up by cells and hydrolyzed to produce an active photosensitizer. Because KI is not taken up by microbial cells, it was of interest to see if aPDI mediated by RBDA could also be potentiated by KI. The addition of 100 mM KI strongly potentiated the killing of Gram-positive methicillin-resistant Staphylocccus aureus, Gram-negative Eschericia coli, and fungal yeast Candida albicans when treated with RBDA (up to 15 µM) for 2 hours followed by green light (540 nm, 10 J/cm). Both RBDA aPDI regimens (400 µM RBDA with or without 400 mM KI followed by 20 J/cm green light) accelerated the healing of MRSA-infected excisional wounds in diabetic mice, without damaging the host tissue.
先前的研究表明，添加无毒的无机盐碘化钾（KI）可以大大增强抗菌光动力灭活（aPDI）的效果。这种方法适用于许多不同的光敏剂，包括在绿光（540nm）激发下的呫吨染料玫瑰红（RB）。二乙酸酯 RB（RBDA）是一种亲脂性 RB 衍生物，很容易被细胞摄取并水解产生活性光敏剂。由于 KI 不会被微生物细胞摄取，因此人们很感兴趣地想知道 RBDA 介导的 aPDI 是否也可以被 KI 增强。当用 RBDA（高达 15µM）处理 2 小时，然后用绿光（540nm，10J/cm）处理时，添加 100mM KI 可大大增强对革兰氏阳性耐甲氧西林金黄色葡萄球菌、革兰氏阴性大肠杆菌和真菌白色念珠菌的杀伤作用。两种 RBDA aPDI 方案（400µM RBDA 加或不加 400mM KI，然后用 20J/cm 绿光）加速了糖尿病小鼠感染 MRSA 的切口伤口的愈合，而不会损伤宿主组织。
