State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
Department of Biology and Agricultural Engineering, Quynhon University, Quynhon, Binhdinh, 590000, Vietnam.
Plant Mol Biol. 2021 Mar;105(4-5):405-417. doi: 10.1007/s11103-020-01096-7. Epub 2021 Jan 2.
We reported that DGS1 plays a positive role in regulating grain size in rice and was regulated by OsBZR1. Grain size is an important agronomic trait that contributes to grain yield. However, the underlying molecular mechanisms that determine final grain size are still largely unknown. We isolated a rice mutant showing reduced grain size in a Co-irradiated variety Nanjing 35 population. We named the mutant decreased grain size1 (dgs1). Map-based cloning and subsequent transgenic CRISPR and complementation assays indicated that a mutation had occurred in LOC_Os03g49900 and that the DGS1 allele regulated grain size. DGS1 encodes a protein with a 7-transmembrane domain and C3HC4 type RING domain. It was widely expressed, especially in young tissues. DGS1 is a membrane-located protein. OsBZR1 (BRASSINAZOLE-RESISTANT1), a core transcription activator of BR signaling, also plays a positive role in grain size. We provided preliminary evidence that OsBZR1 can bind to the DGS1 promoter to activate expression of DGS1.
我们报道了 DGS1 在调控水稻粒长中发挥正向作用，且受 OsBZR1 调控。粒长是一个重要的农艺性状，决定着粒重。然而，决定最终粒长的潜在分子机制在很大程度上仍不清楚。我们从 Co-irradiated 品种南京 35 群体中分离到一个粒长变小的水稻突变体，将其命名为 decreased grain size1（dgs1）。图位克隆和随后的转基因 CRISPR 和互补实验表明 LOC_Os03g49900 发生了突变，DGS1 等位基因调控粒长。DGS1 编码一个具有 7 次跨膜结构域和 C3HC4 型 RING 结构域的蛋白，在水稻中广泛表达，尤其是在幼嫩组织中。DGS1 是一种定位于膜上的蛋白。OsBZR1（BRASSINAZOLE-RESISTANT1）是 BR 信号的核心转录激活因子，在粒长调控中也发挥正向作用。我们初步证明 OsBZR1 可以结合 DGS1 启动子，激活 DGS1 的表达。
