Postgraduate Program in Dentistry, Orthodontics, School of Life Sciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil.
Postgraduate Program in Dentistry, Stomatology and Biosciences, School of Life Sciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil.
Am J Orthod Dentofacial Orthop. 2021 Feb;159(2):193-201. doi: 10.1016/j.ajodo.2019.12.022. Epub 2020 Dec 31.
This study was performed to evaluate the effect of isotretinoin on tooth displacement and tissues related to induced tooth movement (ITM) in rats.
Wistar rats were randomly divided into 4 groups: vegetable oil (O; n = 40), 7.5 mg/kg isotretinoin (I; n = 40), vegetable oil + ITM (OM; n = 44), and 7.5 mg/kg isotretinoin and ITM (IM; n = 39). After the daily application of the solutions for 30 days, an orthodontic appliance was installed to mesially displace the maxillary first right molar (30 cN) of rats in the OM and IM groups. The animals were killed 2, 7, 14, or 21 days after placement of the devices. The animals in the O and I groups did not undergo ITM but were killed simultaneously. The animals were examined for tooth displacement, the neoformation of mature collagen, bone and root resorption, the presence of hyalinized areas, and trabecular bone modeling by microcomputed tomography.
There was no difference in tooth displacement, the number of osteoclasts, the presence of hyalinized areas, or trabecular bone among the O, I, OM, and IM groups across the periods tested (P >0.05). A lower percentage of mature collagen was found in the IM group than in the OM group on day 7 (P <0.05). A lower frequency of root resorption was found in the IM group than in the OM group on days 2 and 21 (P <0.05).
Isotretinoin at 7.5 mg/kg decreased root resorption in rats subjected to ITM.
本研究旨在评估异维 A 酸对大鼠牙齿移位和与诱导牙齿移动（ITM）相关组织的影响。
Wistar 大鼠随机分为 4 组：植物油（O；n=40）、7.5mg/kg 异维 A 酸（I；n=40）、植物油+ITM（OM；n=44）和 7.5mg/kg 异维 A 酸和 ITM（IM；n=39）。在每天应用溶液 30 天后，在 OM 和 IM 组的大鼠上颌第一右侧磨牙（30cN）上安装正畸矫治器以向近中方向移位。在放置器械后 2、7、14 或 21 天处死动物。O 和 I 组的动物未进行 ITM，但同时处死。通过 microCT 检查牙齿移位、成熟胶原的新生、骨和根吸收、玻璃样变区域的存在以及小梁骨建模来评估动物。
在整个测试期间，O、I、OM 和 IM 组之间的牙齿移位、破骨细胞数量、玻璃样变区域的存在或小梁骨无差异（P>0.05）。在第 7 天，IM 组的成熟胶原百分比低于 OM 组（P<0.05）。在第 2 和第 21 天，IM 组的根吸收频率低于 OM 组（P<0.05）。
7.5mg/kg 的异维 A 酸可减少接受 ITM 的大鼠的根吸收。
