Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia.
Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Centre for Translational Medicine, 14 Medical Drive, 117599, Singapore.
Anal Biochem. 2021 Mar 1;616:114098. doi: 10.1016/j.ab.2020.114098. Epub 2020 Dec 31.
A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 °C, 58 °C and 72 °C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 × 10 DNA copies/μL could be significantly and consistently detected above the background emission of the fluorescence signal intensity.
本文展示了一种在无人机上进行聚合酶链反应（PCR）的系统，该系统利用保持在 94°C、58°C 和 72°C 的热块上的毛细管的空间位移，对应于变性、退火和延伸的循环温度。这里使用缩醛作为热块材料，减少了热损失和维持所需温度所需的输入功率（在 18.5 W 以内）。测试表明，浓度低至 1.16×10 DNA 拷贝/μL 的样本可以在荧光信号强度的背景发射之上显著且一致地检测到。
