Department of Life Sciences, University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST). 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam.
Agricultural Genetics Institute (AGI). Km2, Pham Van Dong, Bac Tu Liem, Hanoi, Viet Nam.
J Plant Physiol. 2021 Feb;257:153340. doi: 10.1016/j.jplph.2020.153340. Epub 2020 Dec 15.
Phosphorus is an essential nutrient for plants that is often in short supply. In rice (Oryza sativa L.), inorganic phosphate (P) deficiency leads to various physiological disorders that consequently affect plant productivity. In this study, a large-scale phenotyping experiment using 160 Vietnamese rice landraces was performed under greenhouse conditions, by employing an alpha lattice design with three replicates, to identify quantitative trait loci (QTLs) associated with plant growth inhibition caused by P deficiency. Rice plantlets were grown for six weeks in the PVC sand column (16 cm diameter × 80 cm height) supplied with P-deficient medium (10 μM P) or full-P Yoshida medium (320 μM P). The effects of P deficiency on the number of crown roots, root length, shoot length, root weight, shoot weight and total weight were studied. From 36 significant markers identified using a genome-wide association study, 21 QTLs associated with plant growth inhibition under P starvation were defined. In total, 158 candidate genes co-located with the defined QTLs were identified. Interestingly, one QTL (qRST9.14) was associated with all three weight-traits. The co-located gene GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 13 was found to be potentially involved in P transport. Understanding the molecular mechanisms of Pi-starvation responses, and identifying the potential QTLs responsible for low-P stress tolerance, will provide valuable information for developing new varieties tolerant of low-P conditions.
磷是植物必需的营养物质，但往往供应不足。在水稻（Oryza sativa L.）中，无机磷（P）缺乏会导致各种生理紊乱，从而影响植物的生产力。在这项研究中，通过采用三重复的α格子设计，在温室条件下对 160 个越南水稻地方品种进行了大规模的表型分析实验，以鉴定与 P 缺乏引起的植物生长抑制相关的数量性状基因座（QTLs）。将水稻幼苗在 PVC 沙柱（直径 16 厘米×80 厘米高）中生长 6 周，用缺 P 介质（10 μM P）或全 P 营养液（320 μM P）供应。研究了 P 缺乏对冠根数量、根长、茎长、根重、茎重和总重的影响。通过全基因组关联研究鉴定了 36 个显著标记，定义了与 P 饥饿下植物生长抑制相关的 21 个 QTL。总共鉴定出与定义的 QTL 共定位的 158 个候选基因。有趣的是，一个 QTL（qRST9.14）与所有三个重量性状都有关。共定位基因 GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 13 被认为可能参与 P 转运。了解 Pi 饥饿反应的分子机制，以及鉴定负责低 P 胁迫耐受的潜在 QTL，将为开发耐受低 P 条件的新品种提供有价值的信息。
