与猪腹泻病相关的病毒群落及中国藏猪中一种布菲亚病毒的基因特征分析
Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China.
作者信息
Zhou Long, Hu Chengzhe, Zhou Qun, Yang Danjiao, Wang Lixuan, Zhang Bin
机构信息
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, P.R. China.
Institute of Animal Science of Ganzi Tibetan Autonomous Prefecture of Sichuan Province, Kangding, 626000, P.R. China.
出版信息
Arch Virol. 2021 Feb;166(2):613-617. doi: 10.1007/s00705-020-04932-9. Epub 2021 Jan 3.
To investigate the viral communities in diarrhoeal faeces of Tibetan pigs, 146 diarrhoeic samples were collected from 16 pigs farms on the Tibetan plateau. Nineteen viruses belonging to eleven viral taxonomic families were identified in a pooled library. Metagenomics analysis revealed that the viruses were mainly small linear and circular DNA viruses. Furthermore, sequences of 10 NS1 genes and two complete genomes of PBuVs were obtained by PCR amplification. Sequence comparisons and phylogenetic analysis showed that the PBuVs from Tibetan pigs displayed more abundant genetic diversity than those from domestic pigs. This is the first description of the faecal viral community in Tibetan pigs associated with diarrhoea.
为了调查藏猪腹泻粪便中的病毒群落，从青藏高原的16个猪场采集了146份腹泻样本。在一个混合文库中鉴定出了属于11个病毒分类科的19种病毒。宏基因组学分析表明，这些病毒主要是小型线性和环状DNA病毒。此外，通过PCR扩增获得了10个NS1基因序列和两个PBuV的完整基因组。序列比较和系统发育分析表明，藏猪的PBuV比家猪的PBuV表现出更丰富的遗传多样性。这是首次对与腹泻相关的藏猪粪便病毒群落进行描述。
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