Escola de Química, Universidade Federal Do Rio de Janeiro, Av. Athos da Silveira Ramos, 149-CT, Bl. E, Ilha Do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil.
Microbial Processes and Interactions, Terra Teaching and Research Centre, University of Liège-Gembloux Agro-Bio Tech, Av. de la Faculté 2B, 5030, Gelmbloux, Belgium.
Bioprocess Biosyst Eng. 2021 Apr;44(4):809-818. doi: 10.1007/s00449-020-02489-0. Epub 2021 Jan 3.
The lipolytic yeast Yarrowia lipolytica produces cell-wall-associated lipases, namely Lip7p and Lip8p, that could have interesting properties as catalyst either in free (released lipase fraction-RLF) or cell-associated (cell-bound lipase fraction-CBLF) forms. Herein, a mixture of waste soybean frying oil, yeast extract and bactopeptone was found to favor the enzyme production. Best parameters for lipase activation and release from the cell wall by means of acoustic wave treatment were defined as: 26 W/cm for 1 min for CBLF and 52 W/cm for 2 min for RLF. Optimal pH and temperature values for lipase activity together with storage conditions were similar for both the free enzyme and cell-associated one: pH 7.0; T = 37 °C; and > 70% residual activity for 60 days at 4, - 4 °C and for 15 days at 30 °C.
脂肪酶酵母解脂耶氏酵母产生细胞壁相关的脂肪酶，即 Lip7p 和 Lip8p，无论是以游离（释放的脂肪酶部分-RLF）还是细胞相关（细胞结合的脂肪酶部分-CBLF）形式作为催化剂，都可能具有有趣的特性。本文发现，混合废大豆油炸油、酵母提取物和细菌蛋白胨有利于酶的产生。通过声波处理从细胞壁中激活和释放脂肪酶的最佳参数定义为：CBLF 为 26 W/cm 持续 1 分钟，RLF 为 52 W/cm 持续 2 分钟。游离酶和细胞相关酶的最适 pH 和温度值以及储存条件相似：pH 值 7.0；T = 37°C；在 4、-4°C 下储存 60 天，在 30°C 下储存 15 天，剩余活性均>70%。
