Fabiszewska Agata Urszula, Kotyrba Danuta, Nowak Dorota
Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159c, 02-787 Warsaw, Poland.
Department of Fermentation Technology, prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology in Warsaw, Rakowiecka Street 36, 02-532 Warsaw, Poland.
Ann Microbiol. 2015;65(3):1495-1503. doi: 10.1007/s13213-014-0988-7. Epub 2014 Oct 11.
Glycerol is considered an important renewable feedstock as well as an undesirable side-product of biodiesel production. The aim of this study was to determine whether supplementing a culture medium with a combination of three different carbon sources (olive oil, glucose and glycerol) would optimize lipase production by the yeast . The optimization experiments were conducted with a statistical approach using the mixture design. Analysis of the response surface revealed that it would be possible to compose a medium in which both an an extracellular lipase activity of 0.1 U/mL and up to 37.5 g/L of pure glycerol could be obtained. An YPO-Gl30 medium consisting of 30 g/L glycerol and 19.2 mL/L olive oil was selected for further investigation. Although a high biomass yield was found in all cultures, the glycerol content of the YPO-Gl30 medium slightly influenced yeast growth, but it did not prolong the duration of the lag phase. The hydrolytic activity of the extracellular lipases produced in YPO-Gl30 medium was satisfactory.
甘油被认为是一种重要的可再生原料，同时也是生物柴油生产中不受欢迎的副产物。本研究的目的是确定在培养基中添加三种不同碳源（橄榄油、葡萄糖和甘油）的组合是否会优化酵母产生脂肪酶的过程。优化实验采用混合设计的统计方法进行。响应面分析表明，可以配制出一种培养基，在其中既能获得0.1 U/mL的细胞外脂肪酶活性，又能获得高达37.5 g/L的纯甘油。选择由30 g/L甘油和19.2 mL/L橄榄油组成的YPO-Gl30培养基进行进一步研究。尽管在所有培养物中都发现了较高的生物量产量，但YPO-Gl30培养基中的甘油含量对酵母生长有轻微影响，但并未延长延迟期的持续时间。在YPO-Gl30培养基中产生的细胞外脂肪酶的水解活性令人满意。
