Department of Joint Surgery, the First Affiliated Hospital of Sun Yat-Sen University, #58 zhongshan 2nd road, Guangzhou, 510080, China.
Department of Joint Surgery, The Third Affiliated Hospital of Southern Medical University, #183 zhongshan road, Guangzhou, 510630, China.
Mol Cell Biochem. 2021 Mar;476(3):1411-1420. doi: 10.1007/s11010-020-04003-2. Epub 2021 Jan 3.
The exosomes derived from chondrogenic stem cells and long non-coding RNAs (lncRNAs) play a key role in cartilage regeneration. Here, we investigated the expression profile of exosomal lncRNAs in chondrogenesis of human adipose derived stem cells (hADSCs). hADSCs were induced to differentiate into chondrocytes in vitro. Exosomes from undifferentiated hADSCs and chondrogenic hADSCs were isolated. LncRNA and mRNA expression profiles in the isolated exosomes were analyzed by RNA sequencing. The resultant data were subjected to gene ontology (GO) terms and KEGG pathway analysis to identify differentially expressed lncRNAs. We identified 23 upregulated and 163 downregulated lncRNAs in exosomes derived from chondrogenic hADSCs compared to that in exosomes from undifferentiated hADSCs. In addition, analysis of mRNA expression data revealed 968 upregulated genes and 572 downregulated genes in exosomes of chondrogenic hADSCs. Lncrna and mRNA expression levels were further validated by qRT-PCR. Differentially expressed lncRNAs and mRNAs were utilized to construct a coding-non-coding gene co-expression network (CNC network). GO terms and KEGG pathway enrichment analysis revealed several significant processes differentially regulated between undifferentiated hADSCs and chondrogenic hADSCs. Taken together, this study revealed the differential expression of exosomal lncRNAs of chondrogenic hADSCs and provided a foundation for future study on the cartilage recovery mechanism of exosomes derived from chondrogenic stem cells.
软骨细胞来源的外泌体和长链非编码 RNA（lncRNA）在软骨再生中发挥关键作用。本研究旨在探讨人脂肪来源干细胞（hADSCs）软骨分化中外泌体 lncRNA 的表达谱。体外诱导 hADSCs 分化为软骨细胞。分离未分化 hADSCs 和软骨分化 hADSCs 的外泌体。通过 RNA 测序分析分离外泌体中的 lncRNA 和 mRNA 表达谱。对差异表达的 lncRNA 进行基因本体论（GO）术语和 KEGG 通路分析。与未分化 hADSCs 的外泌体相比，软骨分化 hADSCs 的外泌体中有 23 个上调和 163 个下调的 lncRNA。此外，分析外泌体中 mRNA 表达数据显示，软骨分化 hADSCs 的外泌体中有 968 个上调基因和 572 个下调基因。通过 qRT-PCR 进一步验证了 lncRNA 和 mRNA 的表达水平。差异表达的 lncRNA 和 mRNA 被用于构建编码非编码基因共表达网络（CNC 网络）。GO 术语和 KEGG 通路富集分析显示，未分化 hADSCs 和软骨分化 hADSCs 之间存在多个显著的差异调控过程。综上所述，本研究揭示了软骨分化 hADSCs 外泌体中 lncRNA 的差异表达，为进一步研究软骨细胞来源的外泌体在软骨再生中的作用机制提供了基础。
