Ferguson A C, Kershnar H E, Collin W K, Stiehm E R
Am J Clin Pathol. 1977 Oct;68(4):499-504. doi: 10.1093/ajcp/68.4.499.
The relationship between delayed cutaneous hypersensitivity to Candida albicans and in-vitro lymphocytic proliferative response using a highly sensitive micro-technic was studied in 26 healthy adult subjects and six children with chronic mucocutaneous candidiasis. The maximum in-vitro lymphocytic proliferative response in adult subjects with cutaneous hypersensitivity, 18.94 +/- 4.16 (SE), was significantly greater than that in those without cutaneous reactions, 2.49 +/- 0.45 (SE) (P less than 0.005). A close correlation was found between cutaneous hypersensitivity (mean diameter of induration at 48 hours) and in-vitro lymphocytic proliferative response (r = 0.73, P less than 0.001). A cutaneous reaction of 5 mm or more of induration after injection of 0.1 ml of 1:100 Candida albicans extract corresponded to an in-vitro lymphocytic proliferative index of 5 or more (P less than 0.005), which supports the previously empiric use of 5 mm of induration as an indicator of intact cellular immunity in clinical practice. In the children with chronic mucocutaneous candidiasis, no consistent relationship between cutaneous hypersensitivity and lymphocytic proliferative response was found. Administration of Levamisole resulted in increased lymphocytic proliferation in vitro, and the development of cutaneous hypersensitivity, suggesting potentiation of cellular immune function.
采用高灵敏度微量技术，对26名健康成人受试者和6名慢性黏膜皮肤念珠菌病患儿进行了白色念珠菌迟发性皮肤超敏反应与体外淋巴细胞增殖反应之间关系的研究。有皮肤超敏反应的成人受试者的最大体外淋巴细胞增殖反应为18.94±4.16（标准误），显著高于无皮肤反应者的2.49±0.45（标准误）（P<0.005）。发现皮肤超敏反应（48小时硬结平均直径）与体外淋巴细胞增殖反应之间存在密切相关性（r = 0.73，P<0.001）。注射0.1 ml 1:100白色念珠菌提取物后硬结5 mm或更大的皮肤反应对应于体外淋巴细胞增殖指数为5或更高（P<0.005），这支持了临床实践中以前将5 mm硬结作为细胞免疫完整指标的经验性应用。在慢性黏膜皮肤念珠菌病患儿中，未发现皮肤超敏反应与淋巴细胞增殖反应之间存在一致关系。左旋咪唑的给药导致体外淋巴细胞增殖增加以及皮肤超敏反应的出现，提示细胞免疫功能增强。
