Moser S A, Domer J E, Mather F J
Infect Immun. 1980 Jan;27(1):140-9. doi: 10.1128/iai.27.1.140-149.1980.
Male CBA/J mice, sensitized by cutaneous inoculation with viable Candida albicans blastospores, were used to study in vivo and in vitro cellular immune responses. Three antigens of C. albicans, viz., a cell wall glycoprotein (GP), a membrane extract (ppt-HEX), and soluble cytoplasmic substances (SCS), were used in vitro in a lymphocyte stimulation assay, whereas the GP and ppt-HEX were used in vivo to detect delayed hypersensitivity by the footpad assay. Delayed hypersensitivity to GP and ppt-HEX was transferred from sensitized donors to naive recipients with peritoneal exudate cells and not with serum. Moreover, the transfer of the reactivity to ppt-HEX was abrogated by the prior treatment of the transfer suspension with anti-theta 1.2 serum and complement. The in vitro lymphocyte response to GP and ppt-HEX correlated qualitatively with the in vivo responses. SCS, a preparation shown to be ineffective in vivo previously, did stimulate lymphocytes from sensitized animals in vitro. The in vitro response to Candida antigens, as well as phytohemagglutinin, was abolished by treatment of the lymphocyte suspension with anti-thymocyte 1.2 serum before assay, whereas anti-immunoglobulin serum had less effect on these responses. The in vivo and in vitro reactivity to the Candida antigens, therefore, was dependent upon viable T-lymphocytes. Preliminary specificity studies were carried out in the lymphocyte stimulation assay, using lymphocytes from mice infected with C. albicans tested against ppt-HEX preparations extracted from two other species of Candida, C. tropicalis and C. guillermondii, and from two other pathogenic yeast forms, Histoplasma capsulatum and Blastomyces dermatitidis. Significant cross-reactivity was observed with C. tropicalis only, a species which is known to be serologically related to C. albicans.
通过皮肤接种活的白色念珠菌芽生孢子致敏的雄性CBA/J小鼠，用于研究体内和体外细胞免疫反应。在淋巴细胞刺激试验中，体外使用了白色念珠菌的三种抗原，即细胞壁糖蛋白（GP）、膜提取物（ppt-HEX）和可溶性细胞质物质（SCS），而在体内使用GP和ppt-HEX通过足垫试验检测迟发型超敏反应。对GP和ppt-HEX的迟发型超敏反应通过腹腔渗出细胞从致敏供体转移至未致敏受体，而非通过血清转移。此外，在用抗θ1.2血清和补体预先处理转移悬液后，对ppt-HEX的反应性转移被消除。体外淋巴细胞对GP和ppt-HEX的反应在质量上与体内反应相关。SCS是一种先前显示在体内无效的制剂，但在体外确实能刺激致敏动物的淋巴细胞。在检测前用抗胸腺细胞1.2血清处理淋巴细胞悬液，可消除体外对念珠菌抗原以及植物血凝素的反应，而抗免疫球蛋白血清对这些反应的影响较小。因此，对念珠菌抗原的体内和体外反应性依赖于活的T淋巴细胞。在淋巴细胞刺激试验中进行了初步的特异性研究，使用感染白色念珠菌的小鼠的淋巴细胞，检测其对从其他两种念珠菌，即热带念珠菌和季也蒙念珠菌，以及另外两种致病性酵母形式，荚膜组织胞浆菌和皮炎芽生菌中提取的ppt-HEX制剂的反应。仅观察到与热带念珠菌有显著的交叉反应，热带念珠菌是一种已知在血清学上与白色念珠菌相关的菌种。
