Engineering Research Center of the Chinese Ministry of Education for Edible and Medicinal Fungi and Jilin Province Medical Fungus Resources and Its Development and Utilization Key Research Laboratory, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
Key Laboratory of Medicinal Fungal Resources and Development and Utilization, Jilin Agricultural University, Changchun, Jilin, China; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin, China.
Int J Med Mushrooms. 2020;22(8):731-741. doi: 10.1615/IntJMedMushrooms.2020035472.
The antidiabetic effect of different doses of water extract (WE) and ethanol extract (EE) was tested on a high-fat diet and streptozotocin (STZ) induced diabetic rats. Parameters were evaluated with normal control (NC), diabetes mellitus control (DM), and metformin (M) groups. In the experiment, nine groups were used with eight rats in each group and three doses of each WE and EE were used, with low, medium, and high doses. The results revealed that the DM group lost a significant amount of weight, whereas the NC group's weight increased throughout the experiment. After treatment with Fomitopsis pinicola, the EE group's weight increased gradually. Liver, kidney, and pancreas weight decreased after STZ injection and returned to normal in EE treated groups. Fasting blood glucose (FBG) levels were observed to be significantly lower after F. pinicola treatment. Serum insulin levels were also restored to normal after mushroom extracts supplementation. Specifically, STZ-induced hyperglycemia was inhibited by high dose EE administration. The biochemical analysis revealed that high-dose EE treatment increased HDL-C and decreased TC, TG, and LDL-C. Results demonstrated that high-dose EE administration protected the organ tissues from oxidative stress by normalizing the antioxidant levels, and CAT, SOD, and GSH-Px suppressed the lethal effect of MDA. The study concluded that F. pinicola EE at the dose 300 mg/kg has a more hypoglycemic, hyperinsulinemic, antioxidant, and antihyperlipidemic effect than NC, DM, and M, and regulates hyperglycemia by increasing insulin secretion.
不同剂量水提物（WE）和醇提物（EE）的抗糖尿病作用在高脂肪饮食和链脲佐菌素（STZ）诱导的糖尿病大鼠中进行了测试。使用正常对照组（NC）、糖尿病对照组（DM）和二甲双胍（M）组来评估参数。在实验中，使用了九组，每组有八只大鼠，每组使用三种剂量的 WE 和 EE，分别为低、中、高剂量。结果表明，DM 组体重明显下降，而 NC 组体重在整个实验过程中增加。用 Fomitopsis pinicola 处理后，EE 组的体重逐渐增加。STZ 注射后肝、肾、胰腺重量下降，在 EE 处理组中恢复正常。用蘑菇提取物处理后，空腹血糖（FBG）水平明显降低。血清胰岛素水平也恢复正常。具体来说，高剂量 EE 给药抑制了 STZ 诱导的高血糖。生化分析表明，高剂量 EE 治疗通过提高 HDL-C 和降低 TC、TG 和 LDL-C 来改善高胆固醇血症。结果表明，高剂量 EE 给药通过使抗氧化剂水平正常化，以及 CAT、SOD 和 GSH-Px 抑制 MDA 的致死作用，保护器官组织免受氧化应激。研究结论是，F. pinicola EE 在 300mg/kg 的剂量下具有比 NC、DM 和 M 更强的降血糖、升高胰岛素、抗氧化和抗高脂血症作用，并通过增加胰岛素分泌来调节高血糖。
