Chen Bo, Ye Diya, Luo Lingling, Liu Weirong, Peng Kerong, Shu Xiaoli, Gu Weizhong, Wang Xiaojun, Xiang Charlie, Jiang Mizu
Gastrointestinal Lab, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child, National Children's Regional Medical Center, Hangzhou, China.
Shaoxing People's Hospital, Shaoxing, China.
Front Pharmacol. 2020 Nov 30;11:588560. doi: 10.3389/fphar.2020.588560. eCollection 2020.
Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of , and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as , and . We believe that adhesive bacteria in children's terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn's disease.
人类与共生细菌相互依存，共同进化了数百万年。这些细菌通过非接触性代谢产物与肠道中的人类宿主进行交流。由于大多数肠道细菌不具有黏附性，它们不会穿透黏液层，也不会直接与上皮细胞（ECs）接触。在这里，我们发现有黏附性细菌附着在儿童回肠末端。并且我们还比较了儿童回肠中非黏附性细菌与黏附性细菌的免疫因子。回肠ECs中与黏附性细菌相关的Th17细胞受到刺激。分泌型免疫球蛋白A（SIgA）反应与小鼠实验中的作用相似。免疫组织化学分析证实，Th17细胞中 、 和 蛋白的表达增加。最后，我们使用16S rRNA基因454焦磷酸测序来分析回肠中黏附性细菌和非黏附性细菌之间细菌群落的差异。有黏附性细菌的回肠显示出黏膜相关细菌增加，如 、 和 。我们认为儿童回肠末端的黏附性细菌与Th17细胞活化增加和肠腔分泌型IgA有关。黏附性细菌紧密附着在儿童回肠末端。它们可能在人类肠道免疫和克罗恩病中发挥重要作用。
