Zhang Bo, Yang Hui-Juan, Yang Ya-Zhou, Zhu Zhen-Zhen, Li Ya-Nan, Qu Dong, Zhao Zheng-Yang
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.
Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China.
Front Plant Sci. 2020 Dec 2;11:608109. doi: 10.3389/fpls.2020.608109. eCollection 2020.
Anthocyanins are responsible for the red pigmentation in the peel of apple ( Borkh.) fruit. Relatively few studies have investigated anthocyanins at the posttranscriptional level. MicroRNAs play an important role in plant growth and development by regulating gene expression at the posttranscriptional level. In this study, mdm-miR828 showed a relatively low expression level during the rapid fruit coloration period. However, the mdm-miR828 expression level increased in the late fruit coloration stage. Overexpression of mdm-miR828 inhibited anthocyanin synthesis in apple and . Dual-luciferase and yeast one-hybrid assays showed that MdMYB1 is capable of binding to the promoter of mdm-MIR828b to promote its expression. The results indicate that mdm-miR828 is involved in a feedback regulatory mechanism associated with anthocyanin accumulation in apple. In addition, mdm-miR828 is involved in the inhibition of anthocyanin accumulation in response to high temperature.
花青素是苹果（Borkh.）果实果皮红色素沉着的原因。相对较少的研究在转录后水平上研究花青素。微小RNA通过在转录后水平调节基因表达在植物生长和发育中发挥重要作用。在本研究中，mdm-miR828在果实快速着色期表达水平相对较低。然而，mdm-miR828表达水平在果实着色后期升高。过表达mdm-miR828抑制苹果中的花青素合成。双荧光素酶和酵母单杂交试验表明，MdMYB1能够结合mdm-MIR828b的启动子以促进其表达。结果表明，mdm-miR828参与了与苹果中花青素积累相关的反馈调节机制。此外，mdm-miR828参与响应高温对花青素积累的抑制作用。
