Perfetto Mark, Kirkham Scotia G, Ayers Michael C, Wei Shuo, Gallagher Jennifer E G
Department of Biology, 53 Campus Drive, University of West Virginia, Morgantown, WV, 26506, USA.
Department of Biological Sciences, University of Delaware, Newark, DE, 19716, USA.
Toxicol Rep. 2020 Dec 24;8:38-43. doi: 10.1016/j.toxrep.2020.12.009. eCollection 2021.
Following chemical spill disasters, it is important to estimate the effects of spilled chemicals on humans and the environment. Here we analyzed the toxicological effects of the coal cleaning chemical, 4-methylcyclohexane methanol (MCHM), which was spilled into the Elk River water supply in 2014. The viability of HEK293 T human cell line cultures and embryos was negatively affected, and the addition of the antioxidants alleviated toxicity with MCHM exposure. Additionally, embryos suffered developmental defects as well as reversible non-responsiveness and melanization defects. The impact MCHM has on HEK293 T cells and points to the importance of continued follow-up studies of this chemical.
在化学品泄漏灾难发生后，评估泄漏化学品对人类和环境的影响至关重要。在此，我们分析了2014年泄漏到埃尔克河供水系统中的洗煤化学品4-甲基环己烷甲醇（MCHM）的毒理学效应。HEK293 T人细胞系培养物和胚胎的活力受到负面影响，添加抗氧化剂可减轻MCHM暴露带来的毒性。此外，胚胎出现发育缺陷以及可逆的无反应性和黑化缺陷。MCHM对HEK293 T细胞的影响表明对该化学品持续进行后续研究的重要性。
