Lakstins Katherine, Arnold Lauren, Gunsch Gilian, Khan Safdar, Moore Sarah, Purmessur Devina
Department of Biomedical Engineering The Ohio State University Columbus Ohio USA.
Department of Biology The Ohio State University Columbus Ohio USA.
JOR Spine. 2020 Aug 12;3(4):e1116. doi: 10.1002/jsp2.1116. eCollection 2020 Dec.
There is a need to further explore mechanisms of cartilage endplate (CEP) degeneration, due to its role in the onset and progression of intervertebral disc degeneration and low back pain. Therefore, the goal of this study was to evaluate structure, matrix composition, and cell phenotype between the human and bovine or canine, both clinically relevant animal models currently used to study the intervertebral disc, CEP. This information may be used in addition to other relevant studies, to help determine optimal animal models for use in studying the role of the CEP in intervertebral disc degeneration and back pain. Endplate structure, matrix composition, cell morphology, and gene expression were evaluated using a picrosirius red/alcian blue and hematoxylin and eosin stain, a dimethylmethylene blue assay, and quantitative reverse transcription polymerase chain reaction. The bovine and canine CEPs were thinner with more rounded cells and thicker bony endplates. The canine CEP contained significantly more sulfated glycosaminoglycans. The bovine CEP demonstrated higher expression of ACAN, COL1, and COL2 and lower expression of T, FBLN1, and collagen X (COLX) compared to the human CEP. The canine CEP had higher COL2 and lower COL1, KRT19, MKX, FBLN1, COLX expression compared to human. These similarities and differences between human and bovine or canine CEP are important to consider when evaluating which animal model is most optimal to use in future studies, interpreting research findings using these animal models and assessing translatability to the human condition.
由于软骨终板（CEP）在椎间盘退变和腰痛的发生及进展中起作用，因此有必要进一步探索其退变机制。所以，本研究的目的是评估人类与牛或犬（目前用于研究椎间盘CEP的两种临床相关动物模型）之间的结构、基质组成和细胞表型。除其他相关研究外，这些信息可能有助于确定用于研究CEP在椎间盘退变和背痛中作用的最佳动物模型。使用天狼星红/阿尔辛蓝和苏木精-伊红染色、二甲基亚甲基蓝测定法以及定量逆转录聚合酶链反应来评估终板结构、基质组成、细胞形态和基因表达。牛和犬的CEP更薄，细胞更圆，骨终板更厚。犬的CEP含有显著更多的硫酸化糖胺聚糖。与人类CEP相比，牛的CEP显示出更高的ACAN、COL1和COL2表达以及更低的T、FBLN1和胶原X（COLX）表达。与人类相比，犬的CEP具有更高的COL2表达以及更低的COL1、KRT19、MKX、FBLN1、COLX表达。在评估哪种动物模型最适合用于未来研究、解释使用这些动物模型的研究结果以及评估向人类情况的可转化性时，考虑人类与牛或犬CEP之间的这些异同点很重要。
