Institute of Environment, Florida International University, Miami, FL, USA.
Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL, 33199, USA.
Bull Environ Contam Toxicol. 2021 Jul;107(1):92-99. doi: 10.1007/s00128-020-03078-3. Epub 2021 Jan 3.
Lipids are excellent biomarkers for assessing coral stress, although staghorn coral data (Acropora cervicornis) is lacking. Lipid extraction is the most critical step in lipidomic assessments, usually performed using carcinogenic solvents. Efficient alternative using less toxic methods, such as the BUME method using butanol and methanol as extraction solvents, have not been applied to coral lipidomics evaluations. Thus, we aimed to develop a lipidomic approach to identify important coral health biomarkers by comparing different solvent mixtures in staghorn corals. Total lipid extraction was equivalent for both tested methods, but due to its efficiency in extracting polar lipids, the BUME method was chosen. It was then applied to different coral masses (0.33-1.00 g), resulting in non-significant differences concerning number of lipid classes and compounds. Therefore, this method can be successfully applied to coral assessments in a climate change context, with the added benefit of low sample masses, lessening coral sampling impacts.
脂质是评估珊瑚压力的极佳生物标志物，尽管鹿角珊瑚（Acropora cervicornis）的数据尚缺乏。脂质提取是脂质组学评估中最关键的步骤，通常使用致癌溶剂进行。尚未将使用毒性较小的方法（例如使用丁醇和甲醇作为提取溶剂的 BUME 方法）的高效替代方法应用于珊瑚脂质组学评估。因此，我们旨在通过比较鹿角珊瑚中不同溶剂混合物来开发一种脂质组学方法，以确定重要的珊瑚健康生物标志物。两种测试方法的总脂质提取量相当，但由于丁醇-甲醇（BUME）方法在提取极性脂质方面的效率更高，因此选择了该方法。然后将其应用于不同的珊瑚块（0.33-1.00 g），结果在脂质类和化合物的数量方面没有显着差异。因此，该方法可以成功应用于气候变化背景下的珊瑚评估，其优点是样品质量低，减轻了对珊瑚采样的影响。
