Ipsen Biopharm Ltd, Unit 9 Ash Road North, Wrexham Industrial Estate, Wrexham, LL13 9UF, UK.
World J Microbiol Biotechnol. 2021 Jan 4;37(1):11. doi: 10.1007/s11274-020-02968-2.
The volumetric oxygen mass transfer coefficient ([Formula: see text]) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched [Formula: see text]. The maximum [Formula: see text] of the BioFlo® 320 reusable bioreactor was 109 h, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in [Formula: see text] values when both bioreactors were compared and thus conclude that scalability based on [Formula: see text] is not viable. The maximum [Formula: see text] of the Eppendorf BioBLU 0.3f single-use reported here was 47 h compared to that of the manufacturer's value of 2500 h, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation.
体积氧传质系数([Formula: see text])是需氧高密度细胞发酵中的一个重要参数，因为在这种发酵中，生长中的微生物对氧气的可用性是一个限制因素。如果要在 Eppendorf BioBLU 0.3f 一次性使用小瓶和 BioFlo® 320 可重复使用生物反应器之间进行放大/缩小规模，那么使用匹配的[Formula: see text]可能会很有挑战性。BioFlo® 320 可重复使用生物反应器的最大[Formula: see text]为 109 小时，大约是 BioBLU 0.3f 一次性使用小瓶的两倍。这里的结果表明，当比较这两种生物反应器时，[Formula: see text]值没有重叠，因此得出结论，基于[Formula: see text]的可扩展性是不可行的。这里报告的 Eppendorf BioBLU 0.3f 一次性使用的最大[Formula: see text]为 47 小时，而制造商的值为 2500 小时，表明相差 53 倍。这种差异归因于制造商用于估计的不可兼容的亚硫酸盐添加方法。
