Johnson Eric T, Dowd Patrick F, Skory Christopher D, Dunlap Christopher A
Crop Bioprotection Research, USDA Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.
Renewable Product Technology Research, USDA Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.
Antonie Van Leeuwenhoek. 2021 Jan;114(1):37-44. doi: 10.1007/s10482-020-01495-2. Epub 2021 Jan 3.
A Gram-stain positive, aerobic, motile, rod-shaped bacterium designated as strain CBP-2801 was isolated as a contaminant from a culture containing maize callus in Peoria, Illinois, United States. The strain is unique relative to other Cohnella species due to its slow growth and reduced number of sole carbon sources. Phylogenetic analysis using 16S rRNA indicated that strain CBP-2801 is a Cohnella bacterium and showed the highest similarity to Cohnella xylanilytica (96.8%). Genome-based phylogeny and genomic comparisons based on average nucleotide identity confirmed the strain to be a novel species of Cohnella. Growth occurs at 15-45 °C (optimum 40 °C), pH 5-7 (optimum pH 6) and with 0-1% NaCl. The predominant fatty acids are anteiso-15:0 and 18:1 ω6c. Genome mining for secondary metabolites identified a putative biosynthetic cluster that encodes for a novel lasso peptide. In addition, this study contributes five new genome assemblies of type strains of Cohnella species, a genus with less than 30% of the type strains sequenced. The DNA G + C content is 58.7 mol %. Based on the phenotypic, phylogenetic and biochemical data strain CBP-2801 represents a novel species, for which the name Cohnella zeiphila sp. nov. is proposed. The type strain is CBP-2801 (= DSM 111598 = ATCC TSD-230).
从美国伊利诺伊州皮奥里亚一种含有玉米愈伤组织的培养物中分离出一株革兰氏染色阳性、需氧、运动、杆状细菌，命名为菌株CBP - 2801，它是一种污染物。该菌株相对于其他科奈氏菌属物种具有独特性，因为其生长缓慢且唯一碳源数量减少。使用16S rRNA进行的系统发育分析表明，菌株CBP - 2801是一种科奈氏菌，与解木聚糖科奈氏菌的相似性最高（96.8%）。基于基因组的系统发育和基于平均核苷酸同一性的基因组比较证实该菌株是科奈氏菌属的一个新物种。该菌株在15 - 45°C（最适温度40°C）、pH 5 - 7（最适pH 6）和0 - 1% NaCl条件下生长。主要脂肪酸为anteiso - 15:0和18:1 ω6c。对次生代谢产物进行基因组挖掘发现了一个推定的生物合成簇，该簇编码一种新型套索肽。此外，本研究还贡献了科奈氏菌属模式菌株的五个新基因组组装序列，该属已测序的模式菌株不到30%。DNA G + C含量为58.7 mol%。基于表型、系统发育和生化数据，菌株CBP - 2801代表一个新物种，为此提出名称为嗜玉米科奈氏菌（Cohnella zeiphila sp. nov.）。模式菌株为CBP - 2801（= DSM 111598 = ATCC TSD - 230）。
