拟柏枝孢属一新种，分离自汉拿山的韩国冷杉（Abies koreana）根际土壤。
Cohnella abietis sp. nov., isolated from Korean fir (Abies koreana) rhizospheric soil of Halla mountain.
机构信息
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.
Department of Bioactive Materials, Chonbuk National University, Jeonju, 54896, Republic of Korea.
出版信息
J Microbiol. 2019 Nov;57(11):953-958. doi: 10.1007/s12275-019-9136-1. Epub 2019 Aug 28.
A strictly aerobic, motile, endospore-forming, rod-shaped bacterium, designated HS21, was isolated from rhizospheric soil of the Korean fir tree (Abies koreana) from Halla mountain on Jeju island, Korea. Growth of strain HS21 was observed at pH 6.0-8.0 (optimum: pH 7.0), 0-2% (w/v) NaCl and 4-30°C (optimum: 25°C). A comparative analysis of 16S rRNA gene sequences showed that strain HS21 was most closely related to Cohnella luojiensis HY-22R (97.6%), followed by C. lupini RLAHU4B (97.4%) and C. collisoli NKM-5 (97.2%). The genome of strain HS21 comprised a circular chromosome of 7,059,027 bp with 44.8% G + C content. The DNA-DNA relatedness values between strain HS21 and C. luojiensis HY-22R and C. lupini RLAHU4B were 18.1% and 13.8%, respectively. The major cellular fatty acids (> 5%) of the isolate were anteiso-C, iso-C, C, and iso-C. The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, and three unidentified aminophospholipids. Based on its phenotypic, phylogenetic, genomic, and chemotaxonomic properties, strain HS21 represents a novel species of the genus Cohnella, for which the name Cohnella abietis sp. nov. is proposed. The type strain is HS21 (= KCTC 43028 = CCTCC AB 2019010).
一株严格需氧、运动、产芽孢的杆状细菌，命名为 HS21，从韩国济州岛汉拿山的韩国冷杉（Abies koreana）根际土壤中分离得到。菌株 HS21 的生长可观察到在 pH6.0-8.0（最佳：pH7.0）、0-2%（w/v）NaCl 和 4-30°C（最佳：25°C）。16S rRNA 基因序列的比较分析表明，菌株 HS21 与 Cohnella luojiensis HY-22R（97.6%）最为密切相关，其次是 C. lupini RLAHU4B（97.4%）和 C. collisoli NKM-5（97.2%）。菌株 HS21 的基因组由一个 7,059,027 bp 的圆形染色体组成，GC 含量为 44.8%。菌株 HS21 与 C. luojiensis HY-22R 和 C. lupini RLAHU4B 的 DNA-DNA 相关值分别为 18.1%和 13.8%。该分离株的主要细胞脂肪酸（>5%）为 anteiso-C、iso-C、C 和 iso-C。存在的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、赖氨酸磷脂酰甘油和三种未鉴定的氨基磷脂。基于其表型、系统发育、基因组和化学分类学特性，菌株 HS21 代表了 Cohnella 属的一个新种，建议命名为 Cohnella abietis sp. nov.。模式菌株为 HS21（=KCTC 43028=CCTCC AB 2019010）。
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