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茉莉酸甲酯对菊花抗蚜性的影响。

[Effect of methyl jasmonate on aphid resistance of chrysanthemum.].

作者信息

Fan Jie, Zhang Xue-Ying, Sun Xian-Zhi, Xu Bing-Yan

机构信息

College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

Shandong China-Japan-Korea Chrysanthemum Cooperative Research Center, Tai'an 271018, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2020 Dec;31(12):4197-4205. doi: 10.13287/j.1001-9332.202012.039.

Abstract

We examined the effects of methyl jasmonate on aphid resistance of Chrysanthemum 'Hangbai'. Leaves of chrysanthemum cuttings were sprayed by methyl jasmonic with different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mmol·L) and then inoculated with the aphids . The following parameters are measured, including protective enzymes activities, defense enzyme activities, osmotic substances, secondary metabolites, and key genes of the jasmonic signaling pathway. The relationship between aphid resistance and the jasmonic signaling pathway in chrysanthemum was probed. The results showed that, under the treatments of all the five concentrations of methyl jasmonic, the activities of leaf protective enzymes and defense enzymes as well as the contents of secondary metabolite were increased in Chrysanthemum, whereas the contents of malondialdehyde and soluble sugar were reduced. Moreover, exogenous methyl jasmonate treatment induced the expression of and , enhanced the content of endogenous jasmonic acid and the aphid resistance of chrysanthemum.

摘要

我们研究了茉莉酸甲酯对菊花‘杭白’抗蚜性的影响。用不同浓度(0.01、0.05、0.1、0.5和1 mmol·L)的茉莉酸甲酯喷洒菊花插条的叶片,然后接种蚜虫。测定了以下参数,包括保护酶活性、防御酶活性、渗透物质、次生代谢产物以及茉莉酸信号通路的关键基因。探讨了菊花抗蚜性与茉莉酸信号通路之间的关系。结果表明,在所有五种浓度的茉莉酸甲酯处理下,菊花叶片保护酶和防御酶的活性以及次生代谢产物的含量均增加,而丙二醛和可溶性糖的含量降低。此外,外源茉莉酸甲酯处理诱导了[具体基因1]和[具体基因2]的表达,提高了内源茉莉酸的含量和菊花的抗蚜性。

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