Department of Molecular Genetics and Biotechnologies, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea.
Nikita Botanical Garden, National Scientific Centre, Russian Academy of Sciences, 298648 Yalta, Crimea.
Int J Mol Sci. 2023 Jul 20;24(14):11690. doi: 10.3390/ijms241411690.
is a widespread pest of that causes significant damage to world floriculture. Chemical insecticides and biological methods of control have a number of disadvantages that can be improved by using oligonucleotide insecticides. In this article, we present, for the first time, the results of using oligonucleotide insecticides, for which the target sequence is an internal transcribed spacer (ITS) in a polycistronic rRNA transcript. The mortality of wingless aphid individuals after a Macsan-11 treatment was recorded at a level of 67.15 ± 3.32% 7 days after a single treatment with a solution at a concentration of 100 ng/μL and 97.38 ± 2.49% 7 days after a double treatment with a solution of the same concentration and a daily interval. The contact use of the control oligonucleotide (ACTG)ACT-11. as well as the oligonucleotide insecticides Macsan-11(3') and Macsan-11(5') was not accompanied by insect mortality. Given the high variability in the internal transcribed spacer, which has proven to be a promising target for the action of oligonucleotide insecticides, it is possible to create selective preparations. This study showed the prospects of ribosomal insect pest genes as targets for the action of olinscides, and also demonstrated the high specificity of such insecticidal agents.
是一种广泛存在的害虫，对世界花卉业造成了重大损害。化学杀虫剂和生物防治方法有许多缺点，可以通过使用寡核苷酸杀虫剂来改善。在本文中，我们首次介绍了使用寡核苷酸杀虫剂的结果，其靶序列是多顺反子 rRNA 转录物中的内部转录间隔区（ITS）。在浓度为 100ng/μL 的溶液单次处理 7 天后，无翅蚜虫个体的死亡率为 67.15±3.32%，在相同浓度的溶液双次处理 7 天后，死亡率为 97.38±2.49%，每日间隔。对照寡核苷酸（ACTG）ACT-11 的接触使用以及寡核苷酸杀虫剂 Macsan-11(3') 和 Macsan-11(5') 的接触使用并没有伴随着昆虫死亡。由于内部转录间隔区的高度变异性，它已被证明是寡核苷酸杀虫剂作用的一个有前途的靶标，因此可以创建选择性制剂。这项研究展示了核糖体昆虫基因作为寡核苷酸作用靶标的前景，并证明了这种杀虫剂的高度特异性。
