Luo Hui, Feng Cheng-Cheng, Yue Zhong-Hui, Zhao Jing-Yi, Tang Zhuo-Lei
College of Life Science and Technology, Harbin Normal University/Heilongjiang Province Key Laboratory of Plant Biology, Harbin 150025, China.
Ying Yong Sheng Tai Xue Bao. 2020 Dec;31(12):4243-4250. doi: 10.13287/j.1001-9332.202012.025.
With the environmental changes and the increases of anthropogenic disturbance, the area and degree of salinization in saline-alkaline lands of Songnen Plain have been increasing with an unprecedented rate. In this study, the effects of restoration of natural vegetation, , and on the enzymatic activities and thermomechanical characteristics of enzyme catalyzed reaction of two oxidoreductases (catalase, polyphenol oxidase) and three hydrolases (alkaline phosphatase, sucrase, urease) were investigated in heavily saline-alkaline soils from western Songnen Plain. The results showed that the activities of those five soil enzyme as well as the activation free energy (Δ) increased with increasing temperature, reaching respective maximum at 40 and 45 ℃. The activation enthalpy (Δ) and entropy (Δ) of soil enzyme did not change with increasing temperature. The temperature coefficient () slightly changed and ranged from 1.05 to 1.36 by every 10 ℃ enhancement of temperature. Compared with the bare land, catalase activity increased in natural vegetation and rehabilitated land, but decreased in and remediation land. The change of Δ of catalase showed a contrary trend with that of enzyme activities, while Δ and Δ increased in the restoration areas of and , and decreased in the restoration of natural vegetation and . The activity of polyphenol oxidase decreased or remained unchanged in all restoration sites, and Δ and Δ decreased in natural vegetation and restoration sites, while remained unchanged in and restoration sites. Δ of polyphenol oxidase reached the maximum at 40 ℃ in each restoration site and decreased or remained unchanged at other temperatures. The activities of three hydrolytic enzymes increased in each restoration site compared with the bare land, and the Δ of the enzymes decreased or remained unchanged in each repaired area, while Δ and Δ varied greatly among the restoration sites. Taken together, significant responses of soil enzyme activity and their thermodynamic characteristics to temperature change and vegetation restoration were detected, which would provide better understanding for the restoration of heavily salinized soil.
随着环境变化和人为干扰的增加，松嫩平原盐碱地的盐碱化面积和程度正以前所未有的速度增加。本研究在松嫩平原西部重度盐碱土中，研究了自然植被恢复、[此处原文缺失部分内容]以及[此处原文缺失部分内容]对两种氧化还原酶（过氧化氢酶、多酚氧化酶）和三种水解酶（碱性磷酸酶、蔗糖酶、脲酶）的酶活性及酶催化反应热机械特性的影响。结果表明，这五种土壤酶的活性以及活化自由能（Δ）均随温度升高而增加，分别在40℃和45℃达到各自最大值。土壤酶的活化焓（Δ）和熵（Δ）不随温度升高而变化。温度系数（）略有变化，每升温10℃，其范围为1.05至1.36。与裸地相比，过氧化氢酶活性在自然植被恢复地和[此处原文缺失部分内容]修复地增加，但在[此处原文缺失部分内容]和[此处原文缺失部分内容]修复地降低。过氧化氢酶的Δ变化趋势与酶活性相反，而在[此处原文缺失部分内容]和[此处原文缺失部分内容]恢复区，Δ和Δ增加，在自然植被恢复地和[此处原文缺失部分内容]恢复地降低。在所有恢复位点，多酚氧化酶活性降低或保持不变，在自然植被和[此处原文缺失部分内容]恢复位点，Δ和Δ降低，而在[此处原文缺失部分内容]和[此处原文缺失部分内容]恢复位点保持不变。在每个恢复位点，多酚氧化酶的Δ在40℃时达到最大值，在其他温度下降低或保持不变。与裸地相比，三种水解酶的活性在每个恢复位点均增加，在每个修复区，酶的Δ降低或保持不变，而Δ和Δ在恢复位点之间变化很大。综上所述，检测到土壤酶活性及其热力学特性对温度变化和植被恢复有显著响应，这将为重度盐碱化土壤的恢复提供更好的理解。
