Walters D E, Speth R C
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman 99164-6520.
J Neurochem. 1988 Mar;50(3):812-7. doi: 10.1111/j.1471-4159.1988.tb02985.x.
Adult male Sprague-Dawley rats were treated with 3-acetylpyridine, a neurotoxin selective for the inferior olivary nucleus. Following treatment, the rats exhibited deficits in locomotor behavior indicative of destruction in the inferior olivary nucleus. The rats were sacrificed 3 weeks later, and the binding of 125I-sarcosine, isoleucine angiotensin II to brain homogenates and slide-mounted sections of brainstem was determined. Treatment with 3-acetylpyridine significantly decreased specific 125I-sarcosine, isoleucine angiotensin II binding in homogenates of posteroventral brainstem (containing the inferior olivary nucleus) by approximately 50%. Homogenates of dorsal brainstem and hypothalamus-thalamus-midbrain showed no significant changes in specific binding. Treatment with 3-acetylpyridine did not significantly alter the radioligand binding affinity which was determined in the hypothalamus-thalamus-midbrain. Autoradiographic analysis of 125I-sarcosine, isoleucine angiotensin II binding in the brainstem sections indicated that specific angiotensin II binding sites in the inferior olivary nucleus were virtually eliminated by the 3-acetylpyridine treatment. In addition, a comparatively small, but significant, decrease in specific 125I-sarcosine, isoleucine angiotensin II binding occurred in the solitary tract nucleus/dorsal vagal motor nucleus complex. These results indicate that specific angiotensin II binding sites in the inferior olivary nucleus occur exclusively on neuronal perikarya and/or dendrites.
成年雄性Sprague-Dawley大鼠用3-乙酰吡啶进行处理，3-乙酰吡啶是一种对下橄榄核具有选择性的神经毒素。处理后，大鼠表现出运动行为缺陷，这表明下橄榄核遭到破坏。3周后处死大鼠，测定125I-肌氨酸、异亮氨酸血管紧张素II与脑匀浆及脑干载玻片切片的结合情况。用3-乙酰吡啶处理后，后腹侧脑干（包含下橄榄核）匀浆中特异性125I-肌氨酸、异亮氨酸血管紧张素II结合显著降低约50%。背侧脑干和下丘脑-丘脑-中脑的匀浆特异性结合无显著变化。3-乙酰吡啶处理未显著改变在下丘脑-丘脑-中脑测定的放射性配体结合亲和力。对脑干切片中125I-肌氨酸、异亮氨酸血管紧张素II结合的放射自显影分析表明，3-乙酰吡啶处理几乎消除了下橄榄核中的特异性血管紧张素II结合位点。此外，在孤束核/迷走神经背运动核复合体中，特异性125I-肌氨酸、异亮氨酸血管紧张素II结合出现了相对较小但显著的降低。这些结果表明，下橄榄核中的特异性血管紧张素II结合位点仅存在于神经元胞体和/或树突上。
