Bodsch W, Coenen H H, Stöcklin G, Takahashi K, Hossmann K A
Max-Planck-Institut für Neurologische Forschung, Abteilung für Experimentelle Neurologie, Köln, F.R.G.
J Neurochem. 1988 Mar;50(3):979-83. doi: 10.1111/j.1471-4159.1988.tb03007.x.
Fluorine-18-labeled ortho or para isomers of L-fluorophenylalanine were used in double-label experiments together with L-[3H]phenylalanine for amino acid incorporation into cerebral proteins of Mongolian gerbil brain. It was demonstrated by qualitative regional comparison of the 18F and 3H autoradiographic images that L-p-[18F]fluorophenylalanine is incorporated into proteins and exhibits a regional cerebral protein synthesis pattern. To a minor extent, L-p-fluorophenyl[3-14C]alanine and L-o-[18F]fluorophenylalanine are hydroxylated in vivo to form labeled tyrosine or tyrosine analogues that are incorporated into cerebral proteins as well. The advantage and validity of the application of L-p-[18F]fluorophenylalanine with positron emission tomography for noninvasive studies of cerebral protein synthesis in humans are evaluated on the basis of an experimental animal approach.
将氟-18标记的L-氟苯丙氨酸的邻位或对位异构体与L-[3H]苯丙氨酸一起用于双标记实验，以研究氨基酸掺入蒙古沙鼠脑的脑蛋白中。通过对18F和3H放射自显影图像进行定性区域比较，证实L-p-[18F]氟苯丙氨酸可掺入蛋白质中，并呈现出区域脑蛋白合成模式。在较小程度上，L-p-氟苯基[3-14C]丙氨酸和L-o-[18F]氟苯丙氨酸在体内被羟基化，形成标记的酪氨酸或酪氨酸类似物，它们也会掺入脑蛋白中。基于实验动物方法，评估了L-p-[18F]氟苯丙氨酸与正电子发射断层扫描技术在人体脑蛋白合成无创研究中的应用优势和有效性。
