Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, People's Republic of China.
Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.
Anal Chem. 2021 Feb 2;93(4):2072-2081. doi: 10.1021/acs.analchem.0c03803. Epub 2021 Jan 4.
Effective and cancer diagnosis is expected to ease the burden of continued increased deaths worldwide. Herein, we proposed viscosity of the tumor microenvironment as a biomarker and further develop a versatile optical agent, for monitoring tumor microenvironmental viscosity alterations to achieve cancer diagnosis, therapeutic effect tracking, and anticancer drug screening. When in highly viscous media, near-infrared signals of are specifically activated, endowing the probe with the capacity of avoiding biological autofluorescence and achieving high signal-to-noise ratio imaging. The results of vascular imaging disclosed higher fluorescence of the blood vessels in the tumor than the normal ones, implying tumors being pointed out with brighter fluorescence. With the assistance of fluorescence imaging technology, achieved identifying cancer with high signal-to-noise ratio imaging. In addition, the capability of to evaluate anticancer drug efficacy with viscosity as a robust biomarker was explored. Furthermore, as a proof of concept, screening of the anticancer drugs is also realized through monitoring of the microenvironmental viscosity fluctuations of the tumor with . Note that this proposed fluorescence imaging method outperforms the clinical hematoxylin and eosin (H&E) staining assay with the advantageous features of and characteristics. We expected that this unique strategy will reinvigorate the continued perfection of the cancer diagnosis systems.
有效且准确的癌症诊断有望缓解全球因癌症死亡人数持续增加的负担。在此，我们提出肿瘤微环境的粘滞度作为一种生物标志物，并进一步开发了一种多功能光学试剂，用于监测肿瘤微环境粘滞度的变化，以实现癌症诊断、治疗效果跟踪和抗癌药物筛选。当处于高粘滞度的介质中时，近红外信号会被特定激活，赋予探针避免生物自发荧光并实现高信噪比成像的能力。血管成像的结果显示，肿瘤血管的荧光强度高于正常血管，表明肿瘤的荧光更亮。借助荧光成像技术，实现了高信噪比成像下的癌症识别。此外，还探索了以粘滞度为稳健生物标志物的方法来评估抗癌药物的疗效。此外，作为概念验证，还通过监测肿瘤微环境粘滞度的变化，利用 实现了抗癌药物的筛选。需要注意的是，与临床使用的苏木精和伊红（H&E）染色法相比，这种荧光成像方法具有的优势，显示出了该方法的准确性和灵敏性。我们期望这种独特的策略将为癌症诊断系统的持续完善提供新的思路。
