Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Cancer Biology Research Center (Key Laboratory of the Ministry of Education), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
J Clin Endocrinol Metab. 2021 Apr 23;106(5):e2334-e2346. doi: 10.1210/clinem/dgaa990.
Although the incidence of papillary thyroid carcinoma (PTC) is significantly higher in females than in males, the prognosis of male PTC is more unfavorable. However, the cause of higher malignancy of PTC in male patients remains unclear.
We conducted our analysis on microarrays datasets, tissue samples from PTC patients and the RNAseq datasets from TCGA with survival data.
We searched all publicly available microarray datasets and performed a genome-wide meta-analysis comparing PTC and normal samples. Gene Ontology analysis was then conducted. The candidate genes were tested by quantitative real-time polymerase chain reaction. The analysis of prognostic value of genes was performed with datasets from The Cancer Genome Atlas.
After meta-analyses, 150 significantly differentially expressed genes (DEGs) were specifically found in male subjects. Gene Ontology analysis of these 150 genes revealed that the viral process was activated. Seven genes involved in the viral process in male patients showed a significantly differential expression between PTC and normal tissue. Survival analysis exhibited that the 7 genes, used in combination, were prognostically valuable and, of them, PSMB1 possessed a conspicuous prognostic value, especially in males.
In this study, we searched all publicly available microarray datasets and conducted a comprehensive analysis to understand the male propensity for higher malignancy. We found that markers of viral infection showed significantly differential expression only in male patients compared with their female counterparts and had a sex-sensitive prognostic value in PTC.
虽然甲状腺乳头状癌（PTC）在女性中的发病率明显高于男性，但男性 PTC 的预后更差。然而，男性 PTC 恶性程度更高的原因尚不清楚。
我们对微阵列数据集、PTC 患者的组织样本以及具有生存数据的 TCGA 的 RNAseq 数据集进行了分析。
我们搜索了所有公开可用的微阵列数据集，并进行了全基因组荟萃分析，比较了 PTC 和正常样本。然后进行了基因本体分析。通过来自癌症基因组图谱的数据集测试了候选基因。对基因预后价值的分析。
经过荟萃分析，在男性患者中发现了 150 个明显差异表达的基因（DEGs）。对这 150 个基因的基因本体分析显示，病毒过程被激活。男性患者中涉及病毒过程的 7 个基因在 PTC 和正常组织之间表现出明显的差异表达。生存分析表明，这 7 个基因联合使用具有预后价值，其中 PSMB1 具有显著的预后价值，尤其是在男性中。
在这项研究中，我们搜索了所有公开可用的微阵列数据集，并进行了全面分析，以了解男性恶性程度更高的原因。我们发现，与女性相比，只有男性患者的病毒感染标志物显示出明显的差异表达，并且在 PTC 中具有性别敏感的预后价值。
