Roux C, Gusse M, Chevaillier P, Dadoune J P
Laboratoire d'Histologie-Embryologie, Faculté de Médecine Broussais-Hôtel Dieu, UER Biomédicale, Paris, France.
J Reprod Fertil. 1988 Jan;82(1):35-42. doi: 10.1530/jrf.0.0820035.
Specific polyclonal antisera have been obtained against total human protamines isolated from purified sperm nuclei. The specificity of antibodies was assessed in immunodotting and immunoblotting assays. In this preliminary report, these specific antibodies were used as probes for in-situ determination of histone to protamine transition during human spermiogenesis. Protamine-containing sites were detected on sections of human testes by light microscopy using the immunoperoxidase technique. As in other mammals, protamines appear and concentrate in condensed nuclei of elongating spermatids, i.e. during the later steps of human spermiogenesis.
已经获得了针对从纯化的精子细胞核中分离出的总人鱼精蛋白的特异性多克隆抗血清。通过免疫斑点和免疫印迹分析评估抗体的特异性。在本初步报告中，这些特异性抗体被用作原位测定人类精子发生过程中组蛋白向鱼精蛋白转变的探针。使用免疫过氧化物酶技术通过光学显微镜在人类睾丸切片上检测到含鱼精蛋白的位点。与其他哺乳动物一样，鱼精蛋白在延长的精子细胞的浓缩细胞核中出现并聚集，即在人类精子发生的后期步骤中。
