Roux C, Mathieson J, Dadoune J P
Laboratoire d'Histologie-Embryologie, Faculté de Médecine Broussais Hôtel-Dieu, Paris.
Bull Assoc Anat (Nancy). 1987 Dec;71(215):65-9.
Specific antisera have been obtained against the purified human sperm protamine HP1b. The specificity of antibodies was assessed in dot-blot and western-blot assays. Antibodies showed high binding to protamine HP1 group. Nevertheless cross-reactivity between HP1 and the other protamines (HP2 - HP3 - HP4) could not be excluded. Immunohistochemical studies revealed that protamines HP1 were detected in nuclei of ejaculated sperm after chromatin decondensation. In testis, they appeared in nuclei of elongating spermatids.
已获得针对纯化的人精子鱼精蛋白HP1b的特异性抗血清。通过斑点印迹和蛋白质印迹分析评估抗体的特异性。抗体与鱼精蛋白HP1组表现出高结合力。然而，不能排除HP1与其他鱼精蛋白（HP2 - HP3 - HP4）之间的交叉反应性。免疫组织化学研究表明，在染色质解聚后的射出精子细胞核中检测到鱼精蛋白HP1。在睾丸中，它们出现在伸长的精子细胞的细胞核中。
