17β-雌二醇和睾酮二元混合物对成年雌性西部拟蚊鱼（Gambusia affinis）的内分泌干扰作用。
Endocrine disrupting effects of binary mixtures of 17β-estradiol and testosterone in adult female western mosquitofish (Gambusia affinis).
机构信息
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
出版信息
Ecotoxicol Environ Saf. 2021 Jan 15;208:111566. doi: 10.1016/j.ecoenv.2020.111566. Epub 2020 Nov 5.
Androgens and estrogens often co-exist in aquatic environments and pose potential risks to fish populations. However, little is known about the endocrine disrupting effects of the mixture of androgens and estrogens in fish. In this study, transcriptional level of target genes related to the hypothalamic-pituitary-gonadal-liver (HPGL) axis, sex hormone level, VTG protein concentration, histology and secondary sex characteristic were assessed in the ovaries and livers of adult female western mosquitofish (Gambusia affinis) exposed to 17β-estradiol (E2), testosterone (T), and mixtures of E2 and T for 91 days. The results showed that the transcriptional expression of cytochrome P450, family 19, subfamily A, polypeptide 1a (Cyp19a1a) was suppressed in the 200 ng/L T treatment and the 50 ng/L E2 + 200 ng/L T treatment in the ovaries. Steroidogenic acute regulatory protein (Star) and Cyp11a1 showed a similar expression pattern in the T treatment to its corresponding T + E2 mixtures. In the ovaries, the concentrations of 17β-estradiol and testosterone were decreased in most treatments compared with the solvent control. VTG protein was induced in all steroid treatment. However, exposure to T or E2 + T mixture did not cause the abnormal cells of the ovaries and livers and an extension of the anal fins in female G. affinis. This study demonstrates that chronic exposure to E2, T and their mixtures affects the transcripts of genes in the HPGL axis, steroid hormone level and VTG protein concentration in the ovaries and livers, but fails to cause the histopathological effect of the ovaries and livers and alter the morphology of the anal fins in G. affinis.
雄激素和雌激素通常共存于水生环境中，对鱼类种群构成潜在风险。然而，人们对鱼类中雄激素和雌激素混合物的内分泌干扰作用知之甚少。在这项研究中，我们评估了暴露于 17β-雌二醇（E2）、睾酮（T）和 E2 和 T 混合物 91 天后，成年雌性西部拟蚊鱼（Gambusia affinis）的下丘脑-垂体-性腺-肝（HPGL）轴相关靶基因的转录水平、性激素水平、VTG 蛋白浓度、组织学和次级性特征。结果表明，Cyp19a1a 在 200ng/L T 处理和 50ng/L E2+200ng/L T 处理中卵巢中的表达受到抑制。类固醇生成急性调节蛋白（Star）和 Cyp11a1 在 T 处理中的表达模式与相应的 T+E2 混合物相似。在卵巢中，与溶剂对照相比，大多数处理中 17β-雌二醇和睾酮的浓度降低。VTG 蛋白在所有类固醇处理中均被诱导。然而，暴露于 T 或 E2+T 混合物并未导致 G. affinis 卵巢和肝脏的异常细胞和尾鳍延长。本研究表明，慢性暴露于 E2、T 和它们的混合物会影响 HPGL 轴、卵巢和肝脏中的类固醇激素水平和 VTG 蛋白浓度的基因转录，但不会导致卵巢和肝脏的组织病理学效应，并改变 G. affinis 的尾鳍形态。
Zotero 插件