Narendranathan R, Lutze L H, Jackson G D, Cooper G N
School of Microbiology, University of New South Wales, Kensington, Australia.
J Med Microbiol. 1988 Feb;25(2):129-38. doi: 10.1099/00222615-25-2-129.
Resistance to growth of Vibrio cholerae at the mucosa of blind intestinal loops developed in rats after intestinal or parenteral exposure to live organisms or other antigenic materials. Simultaneous serological studies suggested that neither serum vibriocidal activity nor intestinal mucus antibodies are likely to provide a direct test of antibacterial immune status. Challenge of rats 4 weeks after one dose of antigen may reveal a form of immunity that is not related to antibodies in the intestine and possibly is analogous to long-term immunity expressed in man following infection with the organism. This immunity has not been attributed to lipopolysaccharide (LPS) antigen and does not appear to involve flagella-associated antigens; involvement of antigens other than LPS, such as protein antigens of the outer membranes of V. cholerae, has not yet been substantiated. Separation of monomeric sub-units of outer-membrane proteins by hydrophobic interaction high pressure liquid chromatography has revealed significant quantitative differences among preparations derived from the common serotypes of the organism. These differences may be sufficient to explain the better protection observed when homologous serotypes were used for immunisation and challenge in the long-term resistance model.
在大鼠经肠道或非肠道途径接触活的霍乱弧菌或其他抗原性物质后，盲肠肠袢黏膜处会产生对霍乱弧菌生长的抵抗力。同时进行的血清学研究表明，血清杀弧菌活性和肠道黏液抗体都不太可能直接检测抗菌免疫状态。在给予一剂抗原4周后对大鼠进行攻击，可能会揭示出一种与肠道抗体无关的免疫形式，并且可能类似于人类感染该病原体后所表现出的长期免疫力。这种免疫力并非归因于脂多糖（LPS）抗原，似乎也不涉及鞭毛相关抗原；LPS以外的抗原，如霍乱弧菌外膜的蛋白质抗原，其参与情况尚未得到证实。通过疏水相互作用高压液相色谱法分离外膜蛋白的单体亚基，已揭示出该生物体常见血清型来源的制剂之间存在显著的数量差异。这些差异可能足以解释在长期抗性模型中使用同源血清型进行免疫和攻击时观察到的更好的保护效果。
