Cooper G N, McNab C E, Jackson G D, Walker P
Aust J Exp Biol Med Sci. 1983 Dec;61 ( Pt 6):713-25. doi: 10.1038/icb.1983.67.
Glutaraldehyde-treated V. cholerae organisms bind firmly to the surfaces of plastic microELISA plates, thus providing a stable immobilized antigen for use in enzyme-linked immunosorbent assays (ELISA). Serum absorption and ELISA-inhibition experiments indicate that, in addition to detecting natural antibodies in normal rat serum, the immobilized antigen may be used to quantitate specific anti-V. cholerae antibodies induced in rats by injection of live organisms. Apart from serotypically specific anti-lipopolysaccharide (LPS) antibodies, the reaction with immobilized organisms seems to involve antibodies common to Inaba and Ogawa serotypes; it is suggested that these antibodies are primarily directed against antigens of the outer membrane protein complex of V. cholerae cells. These findings have led to the development of an indirect ELISA method which quantitates levels of antibodies that react with heat-sensitive surface antigens of V. cholerae without involvement of anti-LPS antibodies. When used in conjunction with the indirect LPS-ELISA, the test has been found to provide a more detailed description of the serum antibody responses of rats to parenteral injections of live V. cholerae than has been reported previously.
经戊二醛处理的霍乱弧菌能牢固地结合在塑料微量酶联免疫吸附测定（ELISA）板的表面，从而为酶联免疫吸附测定提供稳定的固定化抗原。血清吸收和ELISA抑制实验表明，除了检测正常大鼠血清中的天然抗体外，固定化抗原还可用于定量注射活菌体后在大鼠体内诱导产生的特异性抗霍乱弧菌抗体。除了血清型特异性抗脂多糖（LPS）抗体外，与固定化菌体的反应似乎涉及稻叶型和小川型血清型共有的抗体；提示这些抗体主要针对霍乱弧菌细胞外膜蛋白复合物的抗原。这些发现促使开发了一种间接ELISA方法，该方法可定量与霍乱弧菌热敏感表面抗原反应的抗体水平，而无需抗LPS抗体的参与。当与间接LPS-ELISA联合使用时，已发现该试验能比以前报道的更详细地描述大鼠经皮下注射活霍乱弧菌后的血清抗体反应。
