Onji Keiko, Kabir Md Arafat, Zhu Bowen, Yokozeki Kenji, Saito Takashi, Akazawa Toshiyuki, Murata Masaru
Division of Clinical Cariology and Endodontology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu-cho, Ishikarigun, Hokkaido 061-0293, Japan.
Division of Oral Regenerative Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu-cho, Ishikarigun, Hokkaido 061-0293, Japan.
Materials (Basel). 2020 Dec 31;14(1):150. doi: 10.3390/ma14010150.
Autologous blood-derived fibrin glue with platelets, called the concentrated growth factor (CGF), can be prepared immediately by only the decided centrifuge without the addition of coagulation factors. Collagen materials combined with recombinant human BMP-2 have been commercially available for clinical use. The fresh CGF is auto-clot with wettability and elasticity, while most collagen membranes are derived from the cow or pig. The fresh CGF has wettability and elasticity, while collagen membranes are dry materials without elasticity. The aim of this study was to observe the microstructures of human CGF membrane and evaluate its behavior as a delivery scaffold of rhBMP-2 in the subcutaneous tissues of nude mice. Twenty-four nude mice (5-week-old, male) were used for the assessment of in vivo ectopic bone formation. Mice were received the CGF membrane as the controls and the CGF/rhBMP-2 membrane as the experimental group in the subcutaneous tissues, and harvested at 7, 10, and 14 days after the graft. Harvested samples were evaluated for the histological examination and the histomorphometric measurement was conducted to compare the residue of the CGF, as well as the new bone. Mature fibrin fibers assembled from multiple fibrillary elements and platelets with the rhBMP-2 membrane induced several bony islands and cartilage without residues of CGF at 14 days, while the CGF membrane alone was almost absorbed at 10 days and failed to induce bone formation at 14 days. These results demonstrated that the fresh, human CGF membrane could contribute to a short-term, sticky fibrin matrix for the delivery of rhBMP-2.
含有血小板的自体血源性纤维蛋白胶，即浓缩生长因子（CGF），仅通过特定的离心机即可立即制备，无需添加凝血因子。与重组人骨形态发生蛋白-2（rhBMP-2）结合的胶原材料已在临床上商业化使用。新鲜的CGF具有自凝性，兼具润湿性和弹性，而大多数胶原膜来源于牛或猪。新鲜的CGF具有润湿性和弹性，而胶原膜是没有弹性的干燥材料。本研究的目的是观察人CGF膜的微观结构，并评估其作为rhBMP-2在裸鼠皮下组织中的递送支架的性能。24只裸鼠（5周龄，雄性）用于评估体内异位骨形成。将CGF膜作为对照组，CGF/rhBMP-2膜作为实验组植入皮下组织，并在移植后7、10和14天进行取材。对取材的样本进行组织学检查，并进行组织形态计量学测量，以比较CGF的残留量以及新骨情况。由多个纤维状成分和血小板组装而成的成熟纤维蛋白纤维与rhBMP-2膜在14天时诱导形成了几个骨岛和软骨，且无CGF残留，而单独的CGF膜在10天时几乎被吸收，在14天时未能诱导骨形成。这些结果表明，新鲜的人CGF膜可为rhBMP-2的递送提供短期的粘性纤维蛋白基质。
