Cold Spring Harb Protoc. 2021 Jan 4;2021(1):2021/1/pdb.prot102160. doi: 10.1101/pdb.prot102160.
is a methylotrophic yeast capable of metabolizing methanol as its sole carbon source. Growth in methanol-containing medium results in dramatic induction of genes in the alcohol oxidation pathway including alcohol oxidase (AOX), formaldehyde dehydrogenase (FLD), and dihydroxyacetone synthase (DHAS). These proteins may comprise up to 30% of the biomass. Investigators have exploited these methanol-dependent genes to generate tightly regulated expression vectors. Most vectors use the strong and tightly regulated promoter to drive heterologous protein expression. Obtaining integrated transformants requires more DNA than transformations into , where the gene is expressed from episomal plasmids; however, transformants are extremely stable and can be stored for many years.
毕赤酵母是一种甲醇营养型酵母，能够将甲醇作为唯一碳源进行代谢。在含有甲醇的培养基中生长会导致醇氧化途径中的基因，包括醇氧化酶（AOX）、甲醛脱氢酶（FLD）和二羟丙酮合酶（DHAS），发生显著诱导。这些蛋白质可能占生物质的 30%。研究人员已经利用这些甲醇依赖性基因来生成严格调控的表达载体。大多数载体使用强而严格调控的启动子来驱动异源蛋白的表达。与转化为酵母不同，获得整合的转化体需要更多的 DNA，因为在酵母中，该基因是从附加体质粒中表达的；然而，转化体非常稳定，可以储存多年。
