左手性 DNA-PAINT 用于改善细胞核中的超分辨率成像。
Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus.
机构信息
Institute of Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin, Germany.
Institute of Virology, Department of Veterinary Science, Freie Universität Berlin, Berlin, Germany.
出版信息
Nat Biotechnol. 2021 May;39(5):551-554. doi: 10.1038/s41587-020-00753-y. Epub 2021 Jan 4.
DNA point accumulation in nanoscale topography (DNA-PAINT) increases the resolution and multiplexing capabilities of super-resolution imaging, but cellular DNA interferes with DNA-DNA hybridization between target and probe in the nucleus. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to natural right-handed DNA (R-DNA) and demonstrate that L-DNA-PAINT has the same specificity and multiplexing capability as R-DNA-PAINT, but improves the imaging of nuclear targets by substantially reducing background signal.
DNA 点积累纳米形貌（DNA-PAINT）提高了超分辨率成像的分辨率和多重成像能力，但细胞 DNA 会干扰细胞核中靶标和探针之间的 DNA-DNA 杂交。在这里，我们引入了不与天然右手 DNA（R-DNA）杂交的左手 DNA（L-DNA）寡聚物，并证明 L-DNA-PAINT 具有与 R-DNA-PAINT 相同的特异性和多重成像能力，但通过显著降低背景信号来改善核靶标的成像。
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