Department of Applied Physics, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.
Analyst. 2021 Feb 21;146(4):1163-1168. doi: 10.1039/d0an01880e. Epub 2021 Jan 5.
We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.
我们使用多模态非线性光学显微镜对人发髓质进行了无标记成像。超多重协同反斯托克斯拉曼散射（CARS）光谱成象清晰地显示了髓内脂质（IMLs）。发现了两组 IMLs：二次谐波产生（SHG）活跃和不活跃。通过将 SHG 分析与 CARS 相结合，这两组分别被鉴定为游离脂肪酸和蜡酯。
