Capitaine Erwan, Moussa Nawel Ould, Louot Christophe, Bardet Sylvia M, Kano Hideaki, Duponchel Ludovic, Lévêque Philippe, Couderc Vincent, Leproux Philippe
XLIM UMR 7252, CNRS-Université de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France.
Department of Applied Physics, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan.
Biomed Opt Express. 2017 Dec 18;9(1):245-253. doi: 10.1364/BOE.9.000245. eCollection 2018 Jan 1.
We present a bimodal imaging system able to obtain epi-detected mutiplex coherent anti-Stokes Raman scattering (M-CARS) and second harmonic generation (SHG) signals coming from biological samples. We studied a fragment of mouse parietal bone and could detect broadband anti-Stokes and SHG responses originating from bone cells and collagen respectively. In addition we compared two post-processing methods to retrieve the imaginary part of the third-order nonlinear susceptibility related to the spontaneous Raman scattering.
我们展示了一种双峰成像系统，该系统能够获取来自生物样本的外差检测多重相干反斯托克斯拉曼散射（M-CARS）和二次谐波产生（SHG）信号。我们研究了小鼠顶骨的一个片段，能够分别检测到源自骨细胞和胶原蛋白的宽带反斯托克斯和SHG响应。此外，我们比较了两种后处理方法，以检索与自发拉曼散射相关的三阶非线性极化率的虚部。
