Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey.
Faculty of Health Sciences, Eastern Mediterranean University, Gazimagusa, TRNC Via Mersin, Turkey.
Arch Microbiol. 2021 May;203(4):1519-1528. doi: 10.1007/s00203-020-02159-5. Epub 2021 Jan 4.
In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C-HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates. The detection of two QS system of the isolates, and eight gene regions consisting of four intact (lasI/R, rhlI/R) and four internal (lasI/R, rhlI/R) genes were tested by PCR assay. According to findings, las and rhI QS system genes were detected in 27 and 30 isolates, respectively, while 3-O-C-HSL was determined in 13 isolates. A total of 22, 27, and 18 isolates were capable of pyocyanin production, protease, and elastase activity, respectively. Biofilm formation was detected using three methods in all 30 isolates: 12 by Congo red agar, 14 by microtiter plate, and 29 by tube test. Twitching and swarming motility types were detected in 30, but the swimming motility was determined in 25 isolates. The rhI QS system genes detected in all of the isolates having three types including motility, PYA production, and protease and elastase activities. The las QS system genes were detected in 27 of the motility, 17 of PYA production, 25 of protease, and 16 of elastase activity having isolates. In conclusion, the high number of P. aeruginosa isolates from fish tested have two QS systems and related virulence factors. There was also correlation between them.
在这项研究中，评估了 30 株鱼源铜绿假单胞菌分离株中的两种群体感应（QS）系统基因 las 和 rhI；N-3-氧代-十二酰基高丝氨酸内酯（AHL；3-O-C-HSL）；以及与它们相关的毒力因子和相关性。通过 PCR 分析检测了分离株的两种 QS 系统和由四个完整（lasI/R、rhlI/R）和四个内部（lasI/R、rhlI/R）基因组成的八个基因区域。结果发现，las 和 rhI QS 系统基因分别在 27 株和 30 株分离株中检测到，而 13 株分离株中检测到 3-O-C-HSL。共有 22、27 和 18 株分离株分别能够产生绿脓菌素、蛋白酶和弹性蛋白酶活性。使用三种方法在所有 30 株分离株中检测到生物膜形成：12 株用刚果红琼脂，14 株用微量滴定板，29 株用管试验。在 30 株分离株中检测到了 twitching 和 swarming 运动类型，但仅在 25 株分离株中检测到了 swimming 运动类型。在所有具有运动、PYA 产生、蛋白酶和弹性蛋白酶活性三种类型的分离株中均检测到 rhI QS 系统基因。在具有运动、PYA 产生、蛋白酶和弹性蛋白酶活性的 27、17、25 和 16 株分离株中分别检测到 las QS 系统基因。总之，从鱼类中检测到的大量铜绿假单胞菌分离株具有两种 QS 系统和相关毒力因子。它们之间也存在相关性。
