Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie 12 Hao, Beijing, People's Republic of China.
Department of Zoology, University of Magway, Magway, Myanmar.
Curr Microbiol. 2021 Feb;78(2):830-836. doi: 10.1007/s00284-020-02323-6. Epub 2021 Jan 4.
During characterization of rhizobacteria, strain DBTS2 was isolated from the rhizosphere soil samples of healthy tomato plants and characterized using a polyphasic taxonomic approach. Phylogenetic analysis using 16S rRNA gene sequences showed this strain belonged to the genus Rhizobium and was most closely related to Rhizobium subbaraonis JC85 (99.1%) and Rhizobium daejeonense CCBAU 10050 (97%). Cells of strain DBTS2 were Gram-negative, short rod, aerobic and non-motile. This novel strain was found to grow at 20-45 °C (optimum 25-37 °C), pH 5-9 (optimum 8) and in the presence of 4% NaCl. It was positive for catalase and oxidase. The predominant cellular fatty acids were Summed Feature 8 (52.7%) and C cyclo ω8c (23.3%). The polar lipids of strain DBTS2 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipid, unidentified aminolipid, four unidentified phospholipids, unidentified lipid, phosphatidylcholine, unknown glycolipid and unknown aminophosphoglycolipids. Q-10 was the major quinone. The DNA-DNA hybridization similarity values between the strain DBTS2 and R. subbaraonis JC85, R. daejeonense CCBAU 10050 and Rhizobium azooxidifex DSM100211 were 46.4%, 20.7% and 25.5%, respectively. The ANI value was 91.96% between strain DBTS2 and R. subbaraonis JC85 and 75.18% between strain DBTS2 and R. daejeonense CCBAU 10050. The DNA G+C content of the genomic DNA was 63.1 mol%. Based on these results, it was concluded that the isolate represents a novel species of the genus Rhizobium, for which the name Rhizobium rhizolycopersici sp. nov. is proposed, with DBTS2 (= CICC 24887 = ACCC61707 = JCM 34245) as the type strain.
在根际细菌的特征描述过程中，从健康番茄植株的根际土壤样本中分离出一株 DBTS2 菌株，并采用多相分类方法进行了表征。16S rRNA 基因序列的系统发育分析表明，该菌株属于根瘤菌属，与 Rhizobium subbaraonis JC85（99.1%）和 Rhizobium daejeonense CCBAU 10050（97%）最为密切相关。菌株 DBTS2 的细胞为革兰氏阴性、短杆状、需氧和非运动性。该新型菌株在 20-45°C（最佳 25-37°C）、pH 5-9（最佳 8）和 4%NaCl 的存在下生长。它对过氧化氢酶和氧化酶呈阳性。主要细胞脂肪酸为 Summed Feature 8（52.7%）和 C 环 ω8c（23.3%）。菌株 DBTS2 的极性脂由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、未鉴定的氨基磷脂、未鉴定的氨基脂、四种未鉴定的磷脂、未鉴定的脂、磷脂酰胆碱、未知的糖脂和未知的氨基糖脂组成。Q-10 是主要的醌。菌株 DBTS2 与 Rhizobium subbaraonis JC85、R. daejeonense CCBAU 10050 和 Rhizobium azooxidifex DSM100211 的 DNA-DNA 杂交相似值分别为 46.4%、20.7%和 25.5%。菌株 DBTS2 与 Rhizobium subbaraonis JC85 的 ANI 值为 91.96%，与 R. daejeonense CCBAU 10050 的 ANI 值为 75.18%。基因组 DNA 的 G+C 含量为 63.1mol%。基于这些结果，得出结论，该分离株代表根瘤菌属的一个新种，建议将其命名为 Rhizobium rhizolycopersici sp. nov.，其模式菌株为 DBTS2（= CICC 24887 = ACCC61707 = JCM 34245）。
