Thin Kyu Kyu, He Shan-Wen, Ma Rong, Wang Xing, Han Ji-Gang, Zhang Xiao-Xia
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Department of Zoology, University of Magway, Magway, 04012, Myanmar.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005698.
A Gram-positive, rod-shaped, motile, endospore-forming strain, DXFW5, was isolated from the rhizosphere soil of tomato. Strain DXFW5 grew at 20-50 °C (optimum, 25-37 °C), pH 5-8 (optimum, pH 7) and in the presence of 3 % NaCl. It was positive for catalase and oxidase. Phylogenetic analysis using 16S rRNA gene sequences showed this strain was most closely related to DSM 16943 (98.0 %) and DSM 22255 (97.4 %). The DNA G+C content was 52.9 mol%. The digital DNA-DNA hybridization values between strain DXFW5 and DSM 16943, DSM 22255 and DSM 24 were 33.1, 24.9 and 21.2 %, respectively. The average nucleotide identity values between strain DXFW5 and DSM 16943 DSM 22255 and DSM 24 were 86.93, 81.77 and 75.98 %, respectively. The major fatty acids were anteiso-C (55.1 %), iso-C (13.2 %) and C (10 %). The polar lipids of strain DXFW5 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine two unidentified phospholipids and three unidentified lipids. MK-7 was the major isoprenoid quinone. Based on these results, it was concluded that the isolate represents a novel species of the genus for which the name sp. nov. is proposed, with DXFW5 (=ACCC 61751=JCM 34488) as the type strain.
从番茄根际土壤中分离出一株革兰氏阳性、杆状、具运动性、产芽孢的菌株DXFW5。菌株DXFW5在20-50°C（最适温度为25-37°C）、pH值5-8（最适pH值为7）以及3% NaCl存在的条件下生长。该菌株过氧化氢酶和氧化酶呈阳性。基于16S rRNA基因序列的系统发育分析表明，该菌株与DSM 16943（相似度98.0%）和DSM 22255（相似度97.4%）关系最为密切。DNA的G+C含量为52.9 mol%。菌株DXFW5与DSM 16943、DSM 22255和DSM 24之间的数字DNA-DNA杂交值分别为33.1%、24.9%和21.2%。菌株DXFW5与DSM 16943、DSM 22255和DSM 24之间的平均核苷酸同一性值分别为86.93%、81.77%和75.98%。主要脂肪酸为anteiso-C（55.1%）、iso-C（13.2%）和C（10%）。菌株DXFW5的极性脂质由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂和三种未鉴定的脂质组成。MK-7是主要的类异戊二烯醌。基于这些结果，得出结论：该分离株代表一个新的属种，为此提出新种名称 sp. nov.，以DXFW5（=ACCC 61751=JCM 34488）作为模式菌株。
