Wang Qiao, Zheng Rui, Sun Xueting, Jiang Ziwei, Yang Fanghui, Lu Qian, Cui Jizhe
College of Life Science and Technology, Harbin Normal University, Harbin 150025, Heilongjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Dec 25;36(12):2657-2673. doi: 10.13345/j.cjb.200381.
To investigate the degradation of polycyclic aromatic hydrocarbons (PAHs) and the changes of rhizosphere microorganisms in the rhizosphere soil of Leymus chinensis during the remediation of PAHs contaminated soil by Comamonas testosteroni (C.t)-assisted Leymus chinensis, we evaluated the removal of PAHs in the rhizosphere of Leymus chinensis using gas chromatography-mass spectrometry (GC-MS), analyzed the bacterial community and the diversity in Leymus chinensis rhizosphere soil by high-throughput sequencing technology, characterized the correlation among PAHs degradation and bacterial community components performing redundancy analysis (RDA) and network analysis, and predicted PAHs degradation potential via PICRUSt software in this paper. The degradation of PAHs in the rhizosphere of Leymus chinensis was promoted, the abundance and diversity of bacteria and the correlation among bacteria and PAHs were changed, and the degradation potential of PAHs in Leymus chinensis rhizosphere soil was enhanced in the later stage of phytoremediation (60-120 d) due to the incorporation of C.t. The accelerated degradation of three PAHs (Nap, Phe, BaP) was accompanied by the differ abundance and correlation of Proteobacteria (Sphingomonas, MND1, Nordella), Actinomycetes (Rubrobacter, Gaiella), Acidobacteria (RB41) and Bacteroides (Flavobacterium) affected by C.t. The results provide new insight into the microorganism choices for microbial assisted plant remediation of soil PAHs and the mechanisms of enhanced PAHs degradation via the combination of Comamonas testosteroni engineering bacteria and plants.
为研究睾丸酮丛毛单胞菌（C.t）辅助羊草修复多环芳烃（PAHs）污染土壤过程中，羊草根际土壤中PAHs的降解及根际微生物的变化，本文采用气相色谱-质谱联用仪（GC-MS）评估羊草根际PAHs的去除情况，利用高通量测序技术分析羊草根际土壤中的细菌群落及其多样性，通过冗余分析（RDA）和网络分析表征PAHs降解与细菌群落组成之间的相关性，并借助PICRUSt软件预测PAHs的降解潜力。结果表明，在植物修复后期（60 - 120 d），由于引入了C.t，促进了羊草根际PAHs的降解，改变了细菌的丰度和多样性以及细菌与PAHs之间的相关性，增强了羊草根际土壤中PAHs的降解潜力。受C.t影响，三种PAHs（萘、菲、苯并[a]芘）的加速降解伴随着变形菌门（鞘氨醇单胞菌属、MND1、诺德氏菌属）、放线菌门（红杆菌属、盖氏菌属）、酸杆菌门（RB41）和拟杆菌门（黄杆菌属）不同的丰度和相关性变化。研究结果为微生物辅助植物修复土壤PAHs的微生物选择以及通过睾丸酮丛毛单胞菌工程菌与植物结合增强PAHs降解的机制提供了新的见解。
