Assessing Microbial Activity and Rhizoremediation in Hydrocarbon and Heavy Metal-Impacted Soil.

Rhizodegradation enhances pollutant degradation through plant-microbe interactions in the rhizosphere. Plant roots provide a colonisation surface and root exudates that promote microbial abundance and activity, facilitating organic pollutant breakdown via direct microbial degradation and co-metabolism. This study assessed the rhizodegradation of weathered petroleum hydrocarbons (PHCs) in heavy metal co-contaminated soil in a microcosm-scale pot trial. Treatments included , , a + mix, and , alongside a non-planted control. After 14 weeks, PHC concentrations were analysed via gas chromatography, and rhizosphere microbial communities were characterised through sequencing. achieved the highest PHC degradation (68%), significantly exceeding the non-planted control ( < 0.05, Kruskal-Wallis test). Hydrocarbon-degrading bacteria, including , , , unclassified sp., and , were enriched in the rhizosphere, with the uncultured clade mle1-27 potentially contributing indirectly. Metals analysis of plant tissues showed that mustard could accumulate copper more than lead and zinc, despite higher concentrations of zinc and lead in the soil. These results highlight the potential of for rhizoremediation in PHC-heavy metal co-contaminated soils.