Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, P. R. China.
University of Chinese Academy of Science, Beijing, P. R. China.
J Sep Sci. 2021 Apr;44(7):1345-1352. doi: 10.1002/jssc.202001080. Epub 2021 Feb 10.
The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products.
缺乏合适的色谱纯化方法使得有效分离传统藏药的化学成分成为挑战。穗花杉是一种研究较少的藏药，据称具有清除自由基的作用。在本工作中，我们利用它作为模型草药，在在线高效液相色谱-1,1-二苯基-2-苦基肼测定的指导下，通过中压色谱和二维反相/反相相互作用液相色谱分离 1,1-二苯基-2-苦基肼抑制剂，并最终从穗花杉提取物中获得两种自由基抑制剂（>95%纯度）。这是首次快速从穗花杉中分离出这些自由基抑制剂的报道。该方法可用于质量标准评估和进一步的药理活性研究，并可作为各种天然产物组成研究的参考。
