Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea.
Program in Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea.
PLoS One. 2021 Jan 5;16(1):e0244635. doi: 10.1371/journal.pone.0244635. eCollection 2021.
Polycystic kidney disease (PKD) is a common genetic disorder that results in a proliferating and enlarging cyst and ultimately leads to loss of kidney function. Because an enlarged cyst is a primary factor for limited kidney function, the large cyst is surgically removed by laparoscopic deroofing or sclerosant. This a relatively nascent treatment method entails complications and sometimes fail due to the cyst fluid refilling and infection. This study proposes using a more stable and effective polidocanol foam with glycerol and Rose Bengal (GRP form) to prevent cyst regeneration and irritation, which is caused by the required body movement during the treatment. Specifically, the foam retention time and viscosity were increased by adding glycerol up to 10% (w/v). The GRP form inhibited cellular proliferation and disrupted cellular junctions, e-cadherin, and cyst formation, demonstrated by the LDH, Live and Dead, and re-plating culture assays. The GRP foam was shown to be a safe and effective treatment as a commercial grade polidocanol foam form by an in vivo study in which subcutaneously injected mice injected with commercial 3% polidocanol, and the GRP foam showed no difference in inflammation. Thus, this study provides an advanced polidocanol form by adding glycerol and Rose-Bengal to help existing sclerotherapy.
多囊肾病（PKD）是一种常见的遗传性疾病，导致囊肿增生和增大，最终导致肾功能丧失。由于增大的囊肿是肾功能有限的主要因素，因此通过腹腔镜去顶或硬化剂来切除大囊肿。这种相对较新的治疗方法存在并发症，有时会因囊肿液再填充和感染而失败。本研究提出使用更稳定有效的聚多卡醇泡沫与甘油和玫瑰红（GRP 形式）来预防因治疗期间所需的身体运动而引起的囊肿再生和刺激。具体来说，通过添加甘油至 10%（w/v）来增加泡沫保留时间和粘度。GRP 形式通过 LDH、活死和再铺板培养测定抑制细胞增殖和破坏细胞连接、E-钙粘蛋白和囊肿形成。体内研究表明，GRP 泡沫作为商业级聚多卡醇泡沫形式是一种安全有效的治疗方法，其中皮下注射小鼠注射商业 3%聚多卡醇，GRP 泡沫在炎症方面没有差异。因此，本研究通过添加甘油和玫瑰红提供了一种先进的聚多卡醇形式，以帮助现有的硬化疗法。
