Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources CNRS UMR 7378, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens, France.
Chemical Engineering and Materials Department, Chemistry College, Complutense University, 28040 Madrid, Spain.
Food Chem. 2021 Jun 1;346:128909. doi: 10.1016/j.foodchem.2020.128909. Epub 2020 Dec 28.
Pectin oligosaccharides, which can be obtained from fruit wastes, have proven their potential as plant immune-system elicitors. Although the precise size of active species is still under investigation, medium size oligosaccharides have been reported as the most active. Three defined oligogalacturonic acid (OGAs) mixtures were produced from commercial pectin, orange peel and apple pomace residues. The methodology developed involves two sequential acid treatments followed by stepwise ethanol precipitation. Without the need of chromatographic separations, three different fractions were obtained. The fractions were analyzed by high performance anion exchange chromatography (HPAEC) and were completely characterized by mass spectrometry, showing that the small size, medium size and large size fractions contained OGAs of degree of polymerization 3 to 9, 6 to 18, and 16 to 55, respectively.
果胶低聚糖可从水果废料中获得，已被证明具有作为植物免疫系统诱导剂的潜力。尽管活性物质的确切大小仍在研究中，但中低聚糖已被报道为最具活性的物质。从商业果胶、橙皮和苹果渣中制备了三种不同的寡半乳糖醛酸（OGA）混合物。所开发的方法涉及两步连续酸处理，然后逐步乙醇沉淀。无需进行色谱分离，就可以得到三种不同的级分。通过高效阴离子交换色谱（HPAEC）对级分进行分析，并通过质谱法对其进行了完全表征，结果表明小、中、大级分分别含有聚合度为 3 至 9、6 至 18 和 16 至 55 的 OGA。
