Rodolakis A, Chancerelle L
Ann Microbiol (Paris). 1977 Jul;128B(1):81-5.
Direct isolation of Chlamydia psittaci (var, ovis) from mouse spleen was performed in parallel by plaque assay on McCoy cells and by staining of cytoplasmic inclusions in HeLa 229 cells. There was a significant difference between the frequency of isolation: the plaque technique was highly sensitive and reproducible; it offers a potential tool for investigations requiring the accurate quantitation of Chlamydiae in tissue sample.
通过在 McCoy 细胞上进行空斑试验以及对 HeLa 229 细胞中的细胞质包涵体进行染色,平行开展了从鼠脾中直接分离鹦鹉热衣原体(羊变种)的操作。分离频率之间存在显著差异:空斑技术高度灵敏且可重复;它为需要对组织样本中的衣原体进行精确定量的研究提供了一种潜在工具。