Escalante-Ochoa C, Rivera-Flores A, Trigo-Tavera F, Romero-Martínez J
Departamento de Microbiología e Inmunología, FMVZ, UNAM.
Rev Latinoam Microbiol. 1996 Jan-Mar;38(1):17-23.
Ovine chlamydiasis is a well known disease in countries with a good practice of breeding sheep herds, not so in Mexico. Aiming to determine Chlamydia psittaci presence in sheep herds in Mexico, we sampled 10 different farmlands in 5 geographical zones on the highlands, gathering a total of 267 viable samples from adult ewes in SPG (92 of them were obtained in a second sampling carried out in five randomly chosen farms). Samples were treated and inoculated on L-929 cells grown in Iscove's supplemented medium; identification of the characteristic intracytoplasmic inclusions was performed by Giemsa stain, and indirect immunofluorescence in suspected samples. Isolation was successful in 92.88% of the trials. No significant differences were observed among the studied herds nor between the two samplings. This high incidence leads to consider seriously the possible pathogenic role of C. psittaci in Mexico.
在养羊业发达的国家，绵羊衣原体病是一种广为人知的疾病，但在墨西哥并非如此。为了确定墨西哥羊群中鹦鹉热衣原体的存在情况，我们在高地的5个地理区域对10个不同的农场进行了采样，从成年母羊中总共采集了267份在特殊蛋白胨葡萄糖肉汤（SPG）中的活样本（其中92份是在随机选择的5个农场进行的第二次采样中获得的）。样本经过处理后接种到在伊斯科夫改良培养基中生长的L-929细胞上；通过吉姆萨染色对特征性的胞质内包涵体进行鉴定，并对疑似样本进行间接免疫荧光检测。92.88%的试验成功分离出病原体。在所研究的畜群之间以及两次采样之间均未观察到显著差异。如此高的发病率使得人们不得不认真考虑鹦鹉热衣原体在墨西哥可能的致病作用。
